Begley TJ~Samson LD, 2002

Pubmed ID 12496357
Title Damage recovery pathways in Saccharomyces cerevisiae revealed by genomic phenotyping and interactome mapping.
Authors Thomas J Begley, Ari S Rosenbach, Trey Ideker, Leona D Samson
Abstract We have generated a genomic phenotyping database identifying hundreds of Saccharomyces cerevisiae genes important for viable cellular recovery after mutagen exposure. Systematic phenotyping of 1,615 gene deletion strains produced distinctive signatures for each of four mutagens. Integration of the phenotyping database with mutagen-induced transcriptional profiling data demonstrated that being transcriptionally responsive to a mutagen does not predict whether or not a gene contributes to recovery from exposure to that mutagen. Computational integration of the database with 4,025 interacting proteins, comprising the yeast interactome, identified several multiprotein networks important for damage recovery. Some networks were associated with DNA metabolism and cell cycle control functions, but most were associated with unexpected functions such as cytoskeleton remodeling, chromatin remodeling, protein, RNA, and lipid metabolism. Hence, a plethora of responses other than the DNA damage response is important for recovery. These network mapping results demonstrate how systematic phenotypic assays may be linked directly to underlying molecular mechanisms.
Citation Mol. Cancer Res. 2002; 1:103-12

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Begley TJ~Samson LD, 2002 growth (spot assay) methyl methanesulfonate [0.01%] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) methyl methanesulfonate [0.02%] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) methyl methanesulfonate [0.025%] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) methyl methanesulfonate [0.03%] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) Di-tert-butyl peroxide [0.25 mM] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) Di-tert-butyl peroxide [0.50 mM] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) Di-tert-butyl peroxide [0.75 mM] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) Di-tert-butyl peroxide [1 mM] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) 4-nitroquinoline N-oxide [0.1 ug/ml] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) 4-nitroquinoline N-oxide [0.2 ug/ml] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) 4-nitroquinoline N-oxide [0.3 ug/ml] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) 4-nitroquinoline N-oxide [0.4 ug/ml] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) ultraviolet light [25 J/m2] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) ultraviolet light [50 J/m2] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) ultraviolet light [75 J/m2] hap a 1,628 Quantitative
Begley TJ~Samson LD, 2002 growth (spot assay) ultraviolet light [100 J/m2] hap a 1,628 Quantitative

Curation history

Data

March 4, 2013 Loaded.

Tested strains

March 4, 2013 Loaded.