Dakshinamurthy A~Garfinkel DJ, 2010

Pubmed ID 20498295
Title BUD22 affects Ty1 retrotransposition and ribosome biogenesis in Saccharomyces cerevisiae.
Authors Arun Dakshinamurthy, Katherine M Nyswaner, Philip J Farabaugh, David J Garfinkel
Abstract A variety of cellular factors affect the movement of the retrovirus-like transposon Ty1. To identify genes involved in Ty1 virus-like particle (VLP) function, the level of the major capsid protein (Gag-p45) and its proteolytic precursor (Gag-p49p) was monitored in a subset of Ty1 cofactor mutants. Twenty-nine of 87 mutants contained alterations in the level of Gag; however, only bud22Delta showed a striking defect in Gag processing. BUD22 affected the +1 translational frameshifting event required to express the Pol proteins protease, integrase, and reverse transcriptase. Therefore, it is possible that the bud22Delta mutant may not produce enough functional Ty1 protease to completely process Gag-p49 to p45. Furthermore, BUD22 is required for 18S rRNA processing and 40S subunit biogenesis and influences polysome density. Together our results suggest that BUD22 is involved in a step in ribosome biogenesis that not only affects general translation, but also may alter the frameshifting efficiency of ribosomes, an event central to Ty1 retrotransposition.
Citation Genetics 2010; 185:1193-205

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Dakshinamurthy A~Garfinkel DJ, 2010 transposable element transposition (Ty1) standard [standard] hap alpha 4,752 Discrete

Curation history

March 26, 2014 Tested strains requested.
March 26, 2014 Tested strains to load.
March 26, 2014 Data waiting for tested.
March 26, 2014 Data to load.
April 30, 2014 Tested strains loaded.
April 30, 2014 Data loaded.