Willingham S~Muchowski PJ, 2003

Pubmed ID 14657499
Title Yeast genes that enhance the toxicity of a mutant huntingtin fragment or alpha-synuclein.
Authors Stephen Willingham, Tiago Fleming Outeiro, Michael J DeVit, Susan L Lindquist, Paul J Muchowski
Abstract Genome-wide screens were performed in yeast to identify genes that enhance the toxicity of a mutant huntingtin fragment or of alpha-synuclein. Of 4850 haploid mutants containing deletions of nonessential genes, 52 were identified that were sensitive to a mutant huntingtin fragment, 86 that were sensitive to alpha-synuclein, and only one mutant that was sensitive to both. Genes that enhanced toxicity of the mutant huntingtin fragment clustered in the functionally related cellular processes of response to stress, protein folding, and ubiquitin-dependent protein catabolism, whereas genes that modified alpha-synuclein toxicity clustered in the processes of lipid metabolism and vesicle-mediated transport. Genes with human orthologs were overrepresented in our screens, suggesting that we may have discovered conserved and nonoverlapping sets of cell-autonomous genes and pathways that are relevant to Huntington's disease and Parkinson's disease.
Citation Science 2003; 302:1769-72

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Willingham S~Muchowski PJ, 2003 growth (spot assay) expression of mutant huntingtin fragment [GAL] hap a ~4,850 Discrete
Willingham S~Muchowski PJ, 2003 growth (spot assay) expression of alpha-sinuclein [unknown] hap a ~4,850 Discrete

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