Kwak YS~Weller DM, 2011

Pubmed ID 21193664
Title Saccharomyces cerevisiae genome-wide mutant screen for sensitivity to 2,4-diacetylphloroglucinol, an antibiotic produced by Pseudomonas fluorescens.
Authors Youn-Sig Kwak, Sangjo Han, Linda S Thomashow, Jennifer T Rice, Timothy C Paulitz, Dongsup Kim, David M Weller
Abstract 2,4-Diacetylphloroglucinol (2,4-DAPG), an antibiotic produced by Pseudomonas fluorescens, has broad-spectrum antibiotic activity, inhibiting organisms ranging from viruses, bacteria, and fungi to higher plants and mammalian cells. The biosynthesis and regulation of 2,4-DAPG in P. fluorescens are well described, but the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a deletion library of Saccharomyces cerevisiae in broth and agar medium supplemented with 2,4-DAPG. We identified 231 mutants that showed increased sensitivity to 2,4-DAPG under both conditions, including 22 multidrug resistance-related mutants. Three major physiological functions correlated with an increase in sensitivity to 2,4-DAPG: membrane function, reactive oxygen regulation, and cell homeostasis. Physiological studies with wild-type yeast validated the results of the mutant screens. The chemical-genetic fitness profile of 2,4-DAPG resembled those of menthol, sodium azide, and hydrogen peroxide determined in previous high-throughput screening studies. Collectively, these findings indicate that 2,4-DAPG acts on multiple basic cellular processes.
Citation Appl. Environ. Microbiol. 2011; 77:1770-6

Datasets

Download the list of datasets
Paper Phenotype Condition Reference Collection Tested mutants Data Details
Kwak YS~Weller DM, 2011 growth (spot assay) 2,4-diacetylphloroglucinol [200 ug/ml] hap a 4,945 Discrete
Kwak YS~Weller DM, 2011 growth (culture turbidity) 2,4-diacetylphloroglucinol [40 ug/ml] hap a 4,945 Quantitative

Curation history

Tested strains

May 19, 2014 Loaded.

Data

March 26, 2014 Request sent.
May 13, 2014 Request sent.
May 15, 2014 Ready to load.
May 19, 2014 Loaded.