Kwak YS~Weller DM, 2011

Pubmed ID 21193664
Title Saccharomyces cerevisiae genome-wide mutant screen for sensitivity to 2,4-diacetylphloroglucinol, an antibiotic produced by Pseudomonas fluorescens.
Authors Youn-Sig Kwak, Sangjo Han, Linda S Thomashow, Jennifer T Rice, Timothy C Paulitz, Dongsup Kim, David M Weller
Abstract 2,4-Diacetylphloroglucinol (2,4-DAPG), an antibiotic produced by Pseudomonas fluorescens, has broad-spectrum antibiotic activity, inhibiting organisms ranging from viruses, bacteria, and fungi to higher plants and mammalian cells. The biosynthesis and regulation of 2,4-DAPG in P. fluorescens are well described, but the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a deletion library of Saccharomyces cerevisiae in broth and agar medium supplemented with 2,4-DAPG. We identified 231 mutants that showed increased sensitivity to 2,4-DAPG under both conditions, including 22 multidrug resistance-related mutants. Three major physiological functions correlated with an increase in sensitivity to 2,4-DAPG: membrane function, reactive oxygen regulation, and cell homeostasis. Physiological studies with wild-type yeast validated the results of the mutant screens. The chemical-genetic fitness profile of 2,4-DAPG resembled those of menthol, sodium azide, and hydrogen peroxide determined in previous high-throughput screening studies. Collectively, these findings indicate that 2,4-DAPG acts on multiple basic cellular processes.
Citation Appl. Environ. Microbiol. 2011; 77:1770-6


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Papers Phenotype Conditions Collection Tested mutants Data Details
Kwak YS~Weller DM, 2011 growth (spot assay) 2,4-diacetylphloroglucinol [200 ug/ml] hap a 4,945 Discrete
Kwak YS~Weller DM, 2011 growth (culture turbidity) 2,4-diacetylphloroglucinol [40 ug/ml] hap a 4,945 Quantitative

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March 26, 2014 Data requested.
May 13, 2014 Data requested.
May 15, 2014 Data to load.
May 19, 2014 Tested strains loaded.
May 19, 2014 Data loaded.