Huang D~Paulovich AG, 2013

Pubmed ID 23382077
Title The preference for error-free or error-prone postreplication repair in Saccharomyces cerevisiae exposed to low-dose methyl methanesulfonate is cell cycle dependent.
Authors Dongqing Huang, Brian D Piening, Amanda G Paulovich
Abstract Cells employ error-free or error-prone postreplication repair (PRR) processes to tolerate DNA damage. Here, we present a genome-wide screen for sensitivity to 0.001% methyl methanesulfonate (MMS). This relatively low dose is of particular interest because wild-type cells exhibit no discernible phenotypes in response to treatment, yet PRR mutants are unique among repair mutants in their exquisite sensitivity to 0.001% MMS; thus, low-dose MMS treatment provides a distinctive opportunity to study postreplication repair processes. We show that upon exposure to low-dose MMS, a PRR-defective rad18Δ mutant stalls into a lengthy G2 arrest associated with the accumulation of single-stranded DNA (ssDNA) gaps. Consistent with previous results following UV-induced damage, reactivation of Rad18, even after prolonged G2 arrest, restores viability and genome integrity. We further show that PRR pathway preference in 0.001% MMS depends on timing and context; cells preferentially employ the error-free pathway in S phase and do not require MEC1-dependent checkpoint activation for survival. However, when PRR is restricted to the G2 phase, cells utilize REV3-dependent translesion synthesis, which requires a MEC1-dependent delay and results in significant hypermutability.
Citation Mol. Cell. Biol. 2013; 33:1515-27

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Huang D~Paulovich AG, 2013 growth (colony size) methylmethane sulphonate [0.001%] hap a 4,905 Discrete

Curation history

July 18, 2013 Tested strains requested.
July 18, 2013 Data requested.
July 18, 2013 Data not available.
July 18, 2013 Data waiting for tested.
July 19, 2013 Tested strains to load.
July 19, 2013 Data to load.
March 15, 2014 Tested strains loaded.
March 15, 2014 Data loaded.