Galvan Marquez I~Smith ML, 2013

Pubmed ID 23624539
Title Disruption of protein synthesis as antifungal mode of action by chitosan.
Authors Imelda Galván Márquez, Jones Akuaku, Isabel Cruz, James Cheetham, Ashkan Golshani, Myron L Smith
Abstract The antimicrobial activity of chitosan has been acknowledged for more than 30 years and yet its mode-of-action remains ambiguous. We analyzed chemical-genetic interactions of low-molecular weight chitosan using a collection of ≈ 4600 S. cerevisiae deletion mutants and found that 31% of the 107 mutants most sensitive to chitosan had deletions of genes related primarily to functions involving protein synthesis. Disruption of protein synthesis by chitosan was substantiated by an in vivo β-galactosidase expression assay suggesting that this is a primary mode of antifungal action. Analysis of the yeast gene deletion array and secondary assays also indicate that chitosan has a minor membrane disruption effect - a leading model of chitosan antimicrobial activity.
Citation Int. J. Food Microbiol. 2013; 164:108-12


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Galvan Marquez I~Smith ML, 2013 growth (colony size) chitosan [1.75 mg/ml] YPD + acetic acid hap a 4,621 Quantitative

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