Samanfar B~Golshani A, 2013

Pubmed ID 23467670
Title Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.
Authors Bahram Samanfar, Katayoun Omidi, Mohsen Hooshyar, Ben Laliberte, Md Alamgir, Andrew J Seal, Eman Ahmed-Muhsin, Duber Frey Viteri, Kamaleldin Said, Firoozeh Chalabian, Ardeshir Golshani, Gabriel Wainer, Daniel Burnside, Kristina Shostak, Magdalena Bugno, William G Willmore, Myron L Smith, Ashkan Golshani
Abstract A genome-wide screen of a yeast non-essential gene-deletion library was used to identify sick phenotypes due to oxygen deprivation. The screen provided a manageable list of 384 potentially novel as well as known oxygen responding (anoxia-survival) genes. The gene-deletion mutants were further assayed for sensitivity to ferrozine and cobalt to obtain a subset of 34 oxygen-responsive candidate genes including the known hypoxic gene activator, MGA2. With each mutant in this subset a plasmid based β-galactosidase assay was performed using the anoxic-inducible promoter from OLE1 gene, and 17 gene deletions were identified that inhibit induction under anaerobic conditions. Genetic interaction analysis for one of these mutants, the RNase-encoding POP2 gene, revealed synthetic sick interactions with a number of genes involved in oxygen sensing and response. Knockdown experiments for CNOT8, human homolog of POP2, reduced cell survival under low oxygen condition suggesting a similar function in human cells.
Citation Mol Biosyst 2013; 9:1351-9

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Samanfar B~Golshani A, 2013 growth (colony size) oxygen [0%] hap a ~4,500 Discrete

Curation history

Data

July 29, 2013 Request sent.
Sept. 23, 2013 Not available.
Oct. 28, 2016 Ready to load.
Jan. 4, 2017 Loaded.

Tested strains

Sept. 23, 2013 Not available.