Samanfar B~Golshani A, 2013

Pubmed ID 23467670
Title Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.
Authors Bahram Samanfar, Katayoun Omidi, Mohsen Hooshyar, Ben Laliberte, Md Alamgir, Andrew J Seal, Eman Ahmed-Muhsin, Duber Frey Viteri, Kamaleldin Said, Firoozeh Chalabian, Ardeshir Golshani, Gabriel Wainer, Daniel Burnside, Kristina Shostak, Magdalena Bugno, William G Willmore, Myron L Smith, Ashkan Golshani
Abstract A genome-wide screen of a yeast non-essential gene-deletion library was used to identify sick phenotypes due to oxygen deprivation. The screen provided a manageable list of 384 potentially novel as well as known oxygen responding (anoxia-survival) genes. The gene-deletion mutants were further assayed for sensitivity to ferrozine and cobalt to obtain a subset of 34 oxygen-responsive candidate genes including the known hypoxic gene activator, MGA2. With each mutant in this subset a plasmid based β-galactosidase assay was performed using the anoxic-inducible promoter from OLE1 gene, and 17 gene deletions were identified that inhibit induction under anaerobic conditions. Genetic interaction analysis for one of these mutants, the RNase-encoding POP2 gene, revealed synthetic sick interactions with a number of genes involved in oxygen sensing and response. Knockdown experiments for CNOT8, human homolog of POP2, reduced cell survival under low oxygen condition suggesting a similar function in human cells.
Citation Mol Biosyst 2013; 9:1351-9


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Samanfar B~Golshani A, 2013 growth (colony size) oxygen [0%] YPD hap a ~4,500 Discrete

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Sept. 23, 2013 Not available.


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