Hirasawa T~Shimizu H, 2013

Pubmed ID 23665193
Title Genome-wide identification of the targets for genetic manipulation to improve L-lactate production by Saccharomyces cerevisiae by using a single-gene deletion strain collection.
Authors Takashi Hirasawa, Masakado Takekuni, Katsunori Yoshikawa, Aki Ookubo, Chikara Furusawa, Hiroshi Shimizu
Abstract To identify genome-wide targets for gene manipulation for increasing L-lactate production in recombinant Saccharomyces cerevisiae strains, we transformed all available single-gene deletion strains of S. cerevisiae with a plasmid carrying the human L-lactate dehydrogenase gene, and examined L-lactate production in the obtained transformants. The thresholds of increased or decreased L-lactate production were determined based on L-lactate production by the standard strain in repetitive experiments. L-lactate production data for 4802 deletion strains were obtained, and deletion strains with increased or decreased L-lactate production were identified. Functional category analysis of genes whose deletion increased L-lactate production revealed that ribosome biogenesis-related genes were overrepresented. Most deletion strains for genes related to ribosome biogenesis exhibited increased L-lactate production in 200-ml batch cultures. We deleted the genes related to ribosome biogenesis in a recombinant strain of S. cerevisiae with a genetic background different from that of the above deletion strains, and examined the effect of target gene deletion on L-lactate production. We observed that deletion of genes related to ribosome biogenesis leads to increased L-lactate production by recombinant S. cerevisiae strains, and the single-gene deletion strain collection could be utilized in identifying target genes for improving L-lactate production in S. cerevisiae recombinant strains.
Citation J. Biotechnol. 2013; 168:185-93

Datasets

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Paper Phenotype Condition Reference Collection Tested mutants Data Details
Hirasawa T~Shimizu H, 2013 L-lactate abundance standard hap alpha 4,687 Quantitative

Curation history

Tested strains

Sept. 18, 2013 Ready to load.
March 15, 2014 Loaded.

Data

Sept. 17, 2013 Clarification needed.
Sept. 18, 2013 Ready to load.
March 15, 2014 Loaded.