Gaytan BD~Vulpe CD, 2013

Pubmed ID 24260565
Title A genome-wide screen identifies yeast genes required for tolerance to technical toxaphene, an organochlorinated pesticide mixture.
Authors Brandon D Gaytán, Alex V Loguinov, Xenia Peñate, Jan-Michael Lerot, Sebastián Chávez, Nancy D Denslow, Chris D Vulpe
Abstract Exposure to toxaphene, an environmentally persistent mixture of chlorinated terpenes previously utilized as an insecticide, has been associated with various cancers and diseases such as amyotrophic lateral sclerosis. Nevertheless, the cellular and molecular mechanisms responsible for these toxic effects have not been established. In this study, we used a functional approach in the model eukaryote Saccharomyces cerevisiae to demonstrate that toxaphene affects yeast mutants defective in (1) processes associated with transcription elongation and (2) nutrient utilization. Synergistic growth defects are observed upon exposure to both toxaphene and the known transcription elongation inhibitor mycophenolic acid (MPA). However, unlike MPA, toxaphene does not deplete nucleotides and additionally has no detectable effect on transcription elongation. Many of the yeast genes identified in this study have human homologs, warranting further investigations into the potentially conserved mechanisms of toxaphene toxicity.
Citation PLoS ONE 2013; 8:e81253

Datasets

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Paper Phenotype Condition Reference Collection Tested mutants Data Details
Gaytan BD~Vulpe CD, 2013 growth (relative abundance in pooled culture) toxaphene [640 uM] hom ~4,607 Quantitative only for hits
Gaytan BD~Vulpe CD, 2013 growth (relative abundance in pooled culture) toxaphene [320 uM] hom ~4,607 Quantitative only for hits
Gaytan BD~Vulpe CD, 2013 growth (relative abundance in pooled culture) toxaphene [160 uM] hom ~4,607 Quantitative only for hits

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Tested strains

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Data

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