Medicherla B~Wolf DH, 2004

Pubmed ID 15167887
Title A genomic screen identifies Dsk2p and Rad23p as essential components of ER-associated degradation.
Authors Balasubrahmanyam Medicherla, Zlatka Kostova, Antje Schaefer, Dieter H Wolf
Abstract We developed a growth test to screen for yeast mutants defective in endoplasmic reticulum (ER) quality control and associated protein degradation (ERAD) using the membrane protein CTL*, a chimeric derivative of the classical ER degradation substrate CPY*. In a genomic screen of approximately 5,000 viable yeast deletion mutants, we identified genes necessary for ER quality control and degradation. Among the new gene products, we identified Dsk2p and Rad23p. We show that these two proteins are probably delivery factors for ubiquitinated ER substrates to the proteasome, following their removal from the membrane via the Cdc48-Ufd1-Npl4p complex. In contrast to the ERAD substrate CTG*, proteasomal degradation of a cytosolic CPY*-GFP fusion is not dependent on Dsk2p and Rad23p, indicating pathway specificity for both proteins. We propose that, in certain degradation pathways, Dsk2p, Rad23p and the trimeric Cdc48 complex function together in the delivery of ubiquitinated proteins to the proteasome, avoiding malfolded protein aggregates in the cytoplasm.
Citation EMBO Rep. 2004; 5:692-7

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Papers Phenotype Conditions Collection Tested mutants Data Details
Medicherla B~Wolf DH, 2004 ER-associated protein degradation ~5,000 None

Curation history

March 21, 2014 Tested strains to request.
March 21, 2014 Data to request.
April 4, 2016 Tested strains requested.
April 4, 2016 Data requested.
June 27, 2016 Tested strains requested.
June 27, 2016 Data requested.
Aug. 16, 2016 Tested strains abandoned.
Aug. 16, 2016 Data abandoned.