Giaever G~Johnston M, 2002

Pubmed ID 12140549
Title Functional profiling of the Saccharomyces cerevisiae genome.
Authors Guri Giaever, Angela M Chu, Li Ni, Carla Connelly, Linda Riles, Steeve Véronneau, Sally Dow, Ankuta Lucau-Danila, Keith Anderson, Bruno André, Adam P Arkin, Anna Astromoff, Mohamed El-Bakkoury, Rhonda Bangham, Rocio Benito, Sophie Brachat, Stefano Campanaro, Matt Curtiss, Karen Davis, Adam Deutschbauer, Karl-Dieter Entian, Patrick Flaherty, Francoise Foury, David J Garfinkel, Mark Gerstein, Deanna Gotte, Ulrich Güldener, Johannes H Hegemann, Svenja Hempel, Zelek Herman, Daniel F Jaramillo, Diane E Kelly, Steven L Kelly, Peter Kötter, Darlene LaBonte, David C Lamb, Ning Lan, Hong Liang, Hong Liao, Lucy Liu, Chuanyun Luo, Marc Lussier, Rong Mao, Patrice Menard, Siew Loon Ooi, Jose L Revuelta, Christopher J Roberts, Matthias Rose, Petra Ross-Macdonald, Bart Scherens, Greg Schimmack, Brenda Shafer, Daniel D Shoemaker, Sharon Sookhai-Mahadeo, Reginald K Storms, Jeffrey N Strathern, Giorgio Valle, Marleen Voet, Guido Volckaert, Ching-yun Wang, Teresa R Ward, Julie Wilhelmy, Elizabeth A Winzeler, Yonghong Yang, Grace Yen, Elaine Youngman, Kexin Yu, Howard Bussey, Jef D Boeke, Michael Snyder, Peter Philippsen, Ronald W Davis, Mark Johnston
Abstract Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically constructed a nearly complete collection of gene-deletion mutants (96% of annotated open reading frames, or ORFs) of the yeast Saccharomyces cerevisiae. DNA sequences dubbed 'molecular bar codes' uniquely identify each strain, enabling their growth to be analysed in parallel and the fitness contribution of each gene to be quantitatively assessed by hybridization to high-density oligonucleotide arrays. We show that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment. Less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal growth in four of the tested conditions. Our results validate the yeast gene-deletion collection as a valuable resource for functional genomics.
Citation Nature 2002; 418:387-91

Datasets

Download the list of datasets
Papers Phenotype Conditions Collection Tested mutants Data Details
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) YPGal [standard], time [15 generations] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) time [15 generations], sorbitol [1.5 M] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) leucine [10%], uracil [2%], histidine [2%], time [15 generations], minimal complete [standard] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) time [15 generations], sodium chloride [1 M] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) pH [8], time [15 generations] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) time [5 generations], YPGal [standard] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) sorbitol [1.5 M], time [5 generations] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) pH [8], time [5 generations] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) sodium chloride [1 M], time [5 generations] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) leucine [10%], histidine [2%], time [5 generations], minimal complete [standard], uracil [2%] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) nystatin [10 uM], time [15 generations] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) nystatin [10 uM], time [5 generations] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) minimal complete [standard] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) minimal complete -Lys [standard] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) minimal complete -Trp [standard] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 growth (relative abundance in pooled culture) minimal complete -Thr [standard] hom 4,739 Quantitative
Giaever G~Johnston M, 2002 cell size YPAD [standard] hom 4,399 Discrete
Giaever G~Johnston M, 2002 elongated cell shape YPAD [standard] hom 4,399 Discrete
Giaever G~Johnston M, 2002 round cell shape YPAD [standard] hom 4,399 Discrete
Giaever G~Johnston M, 2002 clumped cell shape YPAD [standard] hom 4,399 Discrete
Giaever G~Johnston M, 2002 football cell shape YPAD [standard] hom 4,399 Discrete

Curation history

Data

Feb. 17, 2016 Data loaded.

Tested strains

Feb. 17, 2016 Tested strains loaded.