Hanway D~Romesberg FE, 2002

Pubmed ID 12149442
Title Previously uncharacterized genes in the UV- and MMS-induced DNA damage response in yeast.
Authors Denise Hanway, Jodie K Chin, Gang Xia, Guy Oshiro, Elizabeth A Winzeler, Floyd E Romesberg
Abstract A competitive growth assay has been used to identify yeast genes involved in the repair of UV- or MMS-induced DNA damage. A collection of 2,827 yeast strains was analyzed in which each strain has a single ORF replaced with a cassette containing two unique sequence tags, allowing for its detection by hybridization to a high-density oligonucleotide array. The hybridization data identify a high percentage of the deletion strains present in the collection that were previously characterized as being sensitive to the DNA-damaging agents. The assay, and subsequent analysis, has been used to identify six genes not formerly known to be involved in the damage response, whose deletion renders the yeast sensitive to UV or MMS treatment. The recently identified genes include three uncharacterized ORFs, as well as genes that encode protein products implicated in ubiquitination, gene silencing, and transport across the mitochondrial membrane. Epistatsis analysis of four of the genes was performed to determine the DNA damage repair pathways in which the protein products function.
Citation Proc. Natl. Acad. Sci. U.S.A. 2002; 99:10605-10

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Hanway D~Romesberg FE, 2002 growth (relative abundance in pooled culture) ultraviolet light [117 J/m2] YPD hom ~2,827 Quantitative only for hits
Hanway D~Romesberg FE, 2002 growth (undefined) methyl methanesulfonate [0.01%] YPD hom ~2,827 Quantitative only for hits
Hanway D~Romesberg FE, 2002 growth (undefined) methyl methanesulfonate [0.001%] YPD hom ~2,827 Quantitative only for hits
Hanway D~Romesberg FE, 2002 growth (relative abundance in pooled culture) ultraviolet light [158 J/m2] YPD hom ~2,827 Quantitative only for hits
Hanway D~Romesberg FE, 2002 growth (relative abundance in pooled culture) ultraviolet light [189 J/m2] YPD hom ~2,827 Quantitative only for hits
Hanway D~Romesberg FE, 2002 growth (relative abundance in pooled culture) ultraviolet light [203 J/m2] YPD hom ~2,827 Quantitative only for hits
Hanway D~Romesberg FE, 2002 growth (relative abundance in pooled culture) ultraviolet light [216 J/m2] YPD hom ~2,827 Quantitative only for hits
Hanway D~Romesberg FE, 2002 growth (relative abundance in pooled culture) ultraviolet light [243 J/m2] YPD hom ~2,827 Quantitative only for hits
Hanway D~Romesberg FE, 2002 growth (relative abundance in pooled culture) ultraviolet light [270 J/m2] YPD hom ~2,827 Quantitative only for hits
Hanway D~Romesberg FE, 2002 growth (exponential growth rate) ultraviolet light [216 J/m2] YPD hom ~2,827 Quantitative only for hits

Curation history

Data

Sept. 20, 2016 To request.
Sept. 22, 2016 Request sent.
April 25, 2017 Request sent.
May 16, 2017 Request sent.
June 23, 2017 Ready to load.
June 29, 2017 Loaded.

Tested strains

Sept. 20, 2016 To request.
Sept. 22, 2016 Request sent.
April 25, 2017 Request sent.
May 16, 2017 Request sent.
June 23, 2017 Request abandoned.