Steinmetz LM~Davis RW, 2002

Pubmed ID 12134146
Title Systematic screen for human disease genes in yeast.
Authors Lars M Steinmetz, Curt Scharfe, Adam M Deutschbauer, Dejana Mokranjac, Zelek S Herman, Ted Jones, Angela M Chu, Guri Giaever, Holger Prokisch, Peter J Oefner, Ronald W Davis
Abstract High similarity between yeast and human mitochondria allows functional genomic study of Saccharomyces cerevisiae to be used to identify human genes involved in disease. So far, 102 heritable disorders have been attributed to defects in a quarter of the known nuclear-encoded mitochondrial proteins in humans. Many mitochondrial diseases remain unexplained, however, in part because only 40-60% of the presumed 700-1,000 proteins involved in mitochondrial function and biogenesis have been identified. Here we apply a systematic functional screen using the pre-existing whole-genome pool of yeast deletion mutants to identify mitochondrial proteins. Three million measurements of strain fitness identified 466 genes whose deletions impaired mitochondrial respiration, of which 265 were new. Our approach gave higher selection than other systematic approaches, including fivefold greater selection than gene expression analysis. To apply these advantages to human disorders involving mitochondria, human orthologs were identified and linked to heritable diseases using genomic map positions.
Citation Nat. Genet. 2002; 31:400-4

Datasets

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Paper Phenotype Condition Reference Collection Tested mutants Data Details
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) standard YPG [3%] het 5,772 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) standard YPE [2%] het 5,744 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) standard YPL het 5,744 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) standard YPDGE het 5,744 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) dinitrophenol [0.1 mM] YPD het 5,766 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) dinitrophenol [0.25 mM] YPD het 5,766 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) dinitrophenol [0.1 mM] YPG [3%] het 5,766 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) dinitrophenol [0.25 mM] YPG [3%] het 5,766 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) standard YPG [3%] hom 4,723 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) standard YPE [2%] hom 4,723 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) standard YPL hom 4,723 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) standard YPDGE hom 4,723 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) dinitrophenol [0.1 mM] YPD hom 4,718 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) dinitrophenol [0.25 mM] YPD hom 4,718 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) dinitrophenol [0.1 mM] YPG [3%] hom 4,718 Quantitative
Steinmetz LM~Davis RW, 2002 growth (relative abundance in pooled culture) dinitrophenol [0.25 mM] YPG [3%] hom 4,718 Quantitative

Curation history

Tested strains

Aug. 30, 2016 Ready to load.
Sept. 9, 2016 Loaded.

Data

Aug. 30, 2016 Ready to load.
Sept. 9, 2016 Loaded.