Deutschbauer AM~Davis RW, 2002

Pubmed ID 12432101
Title Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae.
Authors Adam M Deutschbauer, Roy M Williams, Angela M Chu, Ronald W Davis
Abstract We have quantitatively monitored the sporulation and germination efficiencies of approximately 4,200 yeast deletion strains in parallel by using a molecular bar coding strategy. In a single study, we doubled the number of genes functionally implicated in sporulation to approximately 400, identifying both positive and negative regulators. Our set of 261 sporulation-deficient genes illustrates the importance of autophagy, carbon utilization, and transcriptional machinery during sporulation. These general cellular factors are more likely to exhibit fitness defects when deleted and less likely to be transcriptionally regulated than sporulation-specific genes. Our postgermination screening assay identified recombinationchromosome segregation genes, aneuploid strains, and possible germination-specific factors. Finally, our results facilitate a genome-wide comparison of expression pattern and mutant phenotype for a developmental process and suggest that 16% of genes differentially expressed during sporulation confer altered efficiency of spore production or defective postgermination growth when disrupted.
Citation Proc. Natl. Acad. Sci. U.S.A. 2002; 99:15530-5


Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Deutschbauer AM~Davis RW, 2002 sporulation efficiency standard sporulation (undefined) hom 4,710 Quantitative only for hits
Deutschbauer AM~Davis RW, 2002 growth (culture turbidity) post-germination YPD hom 4,505 Quantitative only for hits

Curation history


Sept. 16, 2016 Waiting for tested.
Aug. 28, 2017 Ready to load.
Feb. 1, 2018 Loaded.

Tested strains

Sept. 16, 2016 To request.
June 29, 2017 Request sent.
Feb. 1, 2018 Loaded.