Game JC~Brown JM, 2003

Pubmed ID 12816519
Title Use of a genome-wide approach to identify new genes that control resistance of Saccharomyces cerevisiae to ionizing radiation.
Authors John C Game, Geoff W Birrell, James A Brown, Toru Shibata, Clelia Baccari, Angela M Chu, Marsha S Williamson, J Martin Brown
Abstract We have used the recently completed set of all homozygous diploid deletion mutants in budding yeast, S. cerevisiae, to screen for new mutants conferring sensitivity to ionizing radiation. In each strain a different open reading frame (ORF) has been replaced with a cassette containing unique 20-mer sequences that allow the relative abundance of each strain in a pool to be determined by hybridization to a high-density oligonucleotide array. Putative radiation-sensitive mutants were identified as having a reduced abundance in the pool of 4,627 individual deletion strains after irradiation. Of the top 33 strains most sensitive to radiation in this assay, 14 contained genes known to be involved in DNA repair. Eight of the remaining deletion mutants were studied. Only one, which deleted for the ORF YDR014W (which we name RAD61), conferred reproducible radiation sensitivity in both the haploid and diploid deletions and had no problem with spore viability when the haploid was backcrossed to wild-type. The rest showed only marginal sensitivity as haploids, and many had problems with spore viability when backcrossed, suggesting the presence of gross aneuploidy or polyploidy in strains initially presumed haploid. Our results emphasize that secondary mutations or deviations from euploidy can be a problem in screening this resource for sensitivity to ionizing radiation.
Citation Radiat. Res. 2003; 160:14-24

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Game JC~Brown JM, 2003 growth (relative abundance in pooled culture) gamma-irradiation [200 Gy] hom ~4,627 Quantitative

Curation history

June 28, 2016 Tested strains requested.
June 28, 2016 Data requested.
June 29, 2016 Tested strains to load.
June 29, 2016 Tested strains loaded.
June 29, 2016 Data to load.
June 29, 2016 Data loaded.