Wright R~Shoemaker DD, 2003

Pubmed ID 12868057
Title Parallel analysis of tagged deletion mutants efficiently identifies genes involved in endoplasmic reticulum biogenesis.
Authors Robin Wright, Mark L Parrish, Emily Cadera, Lynnelle Larson, Clinton K Matson, Philip Garrett-Engele, Chris Armour, Pek Yee Lum, Daniel D Shoemaker
Abstract Increased levels of HMG-CoA reductase induce cell type- and isozyme-specific proliferation of the endoplasmic reticulum. In yeast, the ER proliferations induced by Hmg1p consist of nuclear-associated stacks of smooth ER membranes known as karmellae. To identify genes required for karmellae assembly, we compared the composition of populations of homozygous diploid S. cerevisiae deletion mutants following 20 generations of growth with and without karmellae. Using an initial population of 1,557 deletion mutants, 120 potential mutants were identified as a result of three independent experiments. Each experiment produced a largely non-overlapping set of potential mutants, suggesting that differences in specific growth conditions could be used to maximize the comprehensiveness of similar parallel analysis screens. Only two genes, UBC7 and YAL011W, were identified in all three experiments. Subsequent analysis of individual mutant strains confirmed that each experiment was identifying valid mutations, based on the mutant's sensitivity to elevated HMG-CoA reductase and inability to assemble normal karmellae. The largest class of HMG-CoA reductase-sensitive mutations was a subset of genes that are involved in chromatin structure and transcriptional regulation, suggesting that karmellae assembly requires changes in transcription or that the presence of karmellae may interfere with normal transcriptional regulation.
Citation Yeast 2003; 20:881-92
Data abstract Deletion collection was screened for genes involved in karmellae (nuclear-associated stacks of smooth ER membrane) assembly.

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Wright R~Shoemaker DD, 2003 growth (pooled culture) expression of HMG-CoA [GAL] hom ~1,557 Unknown
Wright R~Shoemaker DD, 2003 ER distribution expression of HMG-CoA [GAL] hom ~1,557 Unknown
Wright R~Shoemaker DD, 2003 ER distribution constitutive HMG-CoA activity hom ~1,557 Unknown
Wright R~Shoemaker DD, 2003 growth (pooled culture, microarray) expression of HMG-CoA [GAL] Rich minimal - Ura, Gal [2%], Suc [2%] hom ~1,557 Unknown
Wright R~Shoemaker DD, 2003 growth (pooled culture, microarray) expression of HMG-CoA [GAL] Rich minimal - Ura, Gal [2%] hom ~1,557 Unknown
Wright R~Shoemaker DD, 2003 growth (pooled culture, microarray) expression of HMG-CoA [TDH3pr] Rich minimal - Ura, Glu [2%] hom ~1,557 Unknown

Curation history

Tested strains

Oct. 22, 2020 To request.
Oct. 29, 2020 Request sent.

Data

Oct. 22, 2020 Waiting for tested.