Davis-Kaplan SR~Kaplan J, 2004

Pubmed ID 14594803
Title Genome-wide analysis of iron-dependent growth reveals a novel yeast gene required for vacuolar acidification.
Authors Sandra R Davis-Kaplan, Diane McVey Ward, Shelly L Shiflett, Jerry Kaplan
Abstract We conducted a genome-wide screen in the budding yeast Saccharomyces cerevisiae of 4,792 homozygous diploid deletions to identify genes that function in iron metabolism. Strains unable to grow on iron-restricted medium contained deletions of genes that encode the structural components of the high affinity iron transport system (FET3, FTR1), the iron-sensing transcription factor AFT1 or genes required for the assembly of the transport system. We also identified genes that were not previously known to play a role in iron metabolism. Deletion of the gene CWH36 resulted in a severe growth defect on iron-limited medium, as well as increased sensitivity to Congo red and calcofluor white. Iron transport studies demonstrated that Deltacwh36 cells have an inability to copper load apoFet3p. Furthermore, Deltacwh36 cells demonstrated additional phenotypes including distorted vacuole morphology and altered kinetics of FM4-64 trafficking. We show that Deltacwh36 cells have a defect in vacuolar acidification through the use of the pH-sensitive dye LysoSensor Green DND-189. In Deltacwh36 cells, the vacuolar H+-ATPase is not assembled and there are reduced levels of at least one subunit of the V0 complex. The open reading frame responsible for the Deltacwh36 phenotypes is YCL005W-A. This gene contains two introns, has homologues in other Saccharomyces strains, and shows weak homology to a component of the vacuolar H+-ATPase found in organisms as diverse as insect and cow.
Citation J. Biol. Chem. 2004; 279:4322-9

Datasets

Download the list of datasets
Papers Phenotype Conditions Collection Tested mutants Data Details
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) iron sulfate [2.5 uM], YPD [standard], temperature [30°C], bathophenanthroline disulfonate [90 μM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPD [standard], iron sulfate [2.5 uM], copper [50 uM], temperature [30°C], bathophenanthroline disulfonate [90 μM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) iron sulfate [2.5 uM], YPD [standard], temperature [30°C], bathophenanthroline disulfonate [90 μM], iron [100 uM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) iron sulfate [5 uM], temperature [30°C], YPD [standard], bathophenanthroline disulfonate [90 μM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C], YPD [standard], bathophenanthroline disulfonate [90 μM], copper [50 uM], iron sulfate [5 uM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) iron sulfate [5 uM], iron [100 uM], bathophenanthroline disulfonate [90 μM], YPD [standard], temperature [30°C] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPGal [standard], temperature [30°C] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPGal [standard], temperature [30°C], copper [50 uM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) iron [100 uM], YPGal [standard], temperature [30°C] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPGE [standard], temperature [30°C] hom 4,678 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C], YPGE [standard], copper [50 uM] hom 4,585 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPGE [standard], temperature [30°C], iron [100 uM] hom 4,678 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC], bathophenanthroline disulfonate [90 μM], YPD [standard], iron sulfate [2.5 uM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPD [standard], bathophenanthroline disulfonate [90 μM], iron sulfate [2.5 uM], temperature [37ºC], copper [50 uM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPD [standard], temperature [37ºC], iron sulfate [2.5 uM], iron [100 uM], bathophenanthroline disulfonate [90 μM] hom 4,652 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPD [standard], iron sulfate [5 uM], bathophenanthroline disulfonate [90 μM], temperature [37ºC] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) iron sulfate [5 uM], YPD [standard], bathophenanthroline disulfonate [90 μM], copper [50 uM], temperature [37ºC] hom 4,558 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) iron [100 uM], temperature [37ºC], bathophenanthroline disulfonate [90 μM], YPD [standard], iron sulfate [5 uM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPGal [standard], temperature [37ºC], copper [50 uM] hom 4,640 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) iron [100 uM], temperature [37ºC], YPGal [standard] hom 4,652 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPGal [standard], temperature [37ºC] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPGE [standard], temperature [37ºC] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPGE [standard], copper [50 uM], temperature [37ºC] hom 4,464 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC], YPGE [standard], iron [100 uM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPD [standard], temperature [30°C] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC], YPD [standard] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPD [standard], copper [50 uM], temperature [30°C] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC], YPD [standard], copper [50 uM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C], YPD [standard], iron [100 uM] hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) YPD [standard], iron [100 uM], temperature [37ºC] hom 4,651 Discrete

Curation history

Sept. 19, 2016 Tested strains to request.
Sept. 19, 2016 Data to request.
Sept. 23, 2016 Tested strains requested.
Sept. 23, 2016 Data requested.
Oct. 19, 2016 Tested strains to load.
Oct. 19, 2016 Data to load.
Nov. 21, 2016 Tested strains loaded.
Nov. 21, 2016 Data loaded.