Davis-Kaplan SR~Kaplan J, 2004

Pubmed ID 14594803
Title Genome-wide analysis of iron-dependent growth reveals a novel yeast gene required for vacuolar acidification.
Authors Sandra R Davis-Kaplan, Diane McVey Ward, Shelly L Shiflett, Jerry Kaplan
Abstract We conducted a genome-wide screen in the budding yeast Saccharomyces cerevisiae of 4,792 homozygous diploid deletions to identify genes that function in iron metabolism. Strains unable to grow on iron-restricted medium contained deletions of genes that encode the structural components of the high affinity iron transport system (FET3, FTR1), the iron-sensing transcription factor AFT1 or genes required for the assembly of the transport system. We also identified genes that were not previously known to play a role in iron metabolism. Deletion of the gene CWH36 resulted in a severe growth defect on iron-limited medium, as well as increased sensitivity to Congo red and calcofluor white. Iron transport studies demonstrated that Deltacwh36 cells have an inability to copper load apoFet3p. Furthermore, Deltacwh36 cells demonstrated additional phenotypes including distorted vacuole morphology and altered kinetics of FM4-64 trafficking. We show that Deltacwh36 cells have a defect in vacuolar acidification through the use of the pH-sensitive dye LysoSensor Green DND-189. In Deltacwh36 cells, the vacuolar H+-ATPase is not assembled and there are reduced levels of at least one subunit of the V0 complex. The open reading frame responsible for the Deltacwh36 phenotypes is YCL005W-A. This gene contains two introns, has homologues in other Saccharomyces strains, and shows weak homology to a component of the vacuolar H+-ATPase found in organisms as diverse as insect and cow.
Citation J. Biol. Chem. 2004; 279:4322-9

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [2.5 uM], temperature [30°C] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [2.5 uM], temperature [30°C], copper [50 uM] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [2.5 uM], temperature [30°C], iron [100 uM] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [5 uM], temperature [30°C] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [5 uM], temperature [30°C], copper [50 uM] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [5 uM], temperature [30°C], iron [100 uM] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C] YPGal hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C], copper [50 uM] YPGal hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C], iron [100 uM] YPGal hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C] YPGE hom 4,678 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C], copper [50 uM] YPGE hom 4,585 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C], iron [100 uM] YPGE hom 4,678 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [2.5 uM], temperature [37ºC] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [2.5 uM], temperature [37ºC], copper [50 uM] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [2.5 uM], temperature [37ºC], iron [100 uM] YPD hom 4,652 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [5 uM], temperature [37ºC] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [5 uM], temperature [37ºC], copper [50 uM] YPD hom 4,558 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate [90 μM], iron sulfate pentahydrate [5 uM], temperature [37ºC], iron [100 uM] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC], copper [50 uM] YPGal hom 4,640 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC], iron [100 uM] YPGal hom 4,652 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC] YPGal hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC] YPGE hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC], copper [50 uM] YPGE hom 4,464 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC], iron [100 uM] YPGE hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C], copper [50 uM] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC], copper [50 uM] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [30°C], iron [100 uM] YPD hom 4,651 Discrete
Davis-Kaplan SR~Kaplan J, 2004 growth (spot assay) temperature [37ºC], iron [100 uM] YPD hom 4,651 Discrete

Curation history

Data

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Tested strains

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