Giaever G~Davis RW, 2004

Pubmed ID 14718668
Title Chemogenomic profiling: identifying the functional interactions of small molecules in yeast.
Authors Guri Giaever, Patrick Flaherty, Jochen Kumm, Michael Proctor, Corey Nislow, Daniel F Jaramillo, Angela M Chu, Michael I Jordan, Adam P Arkin, Ronald W Davis
Abstract We demonstrate the efficacy of a genome-wide protocol in yeast that allows the identification of those gene products that functionally interact with small molecules and result in the inhibition of cellular proliferation. Here we present results from screening 10 diverse compounds in 80 genome-wide experiments against the complete collection of heterozygous yeast deletion strains. These compounds include anticancer and antifungal agents, statins, alverine citrate, and dyclonine. In several cases, we identified previously known interactions; furthermore, in each case, our analysis revealed novel cellular interactions, even when the relationship between a compound and its cellular target had been well established. In addition, we identified a chemical core structure shared among three therapeutically distinct compounds that inhibit the ERG24 heterozygous deletion strain, demonstrating that cells may respond similarly to compounds of related structure. The ability to identify on-and-off target effects in vivo is fundamental to understanding the cellular response to small-molecule perturbants.
Citation Proc. Natl. Acad. Sci. U.S.A. 2004; 101:793-8

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) methotrexate [125 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) methotrexate [250 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) methotrexate [500 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) 5-fluorouracil [4.8 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) 5-fluorouracil [9.6 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) 5-fluorouracil [19.2 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) cisplatin [62.5 uM], time [o/n + 20 gen] YPD hom 4,683 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) 5-fluorouracil [38.5 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) 5-fluorouracil [76.9 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) cisplatin [125 uM], time [o/n + 20 gen] YPD hom 4,683 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) cisplatin [250 uM], time [o/n + 20 gen] YPD hom 4,683 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) cisplatin [500 uM], time [o/n + 20 gen] YPD hom 4,683 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) cisplatin [31.25 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) cisplatin [62.5 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) cisplatin [125 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) time [o/n + 20 gen], atorvastatin [62.5 uM] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) time [o/n + 20 gen], atorvastatin [125 uM] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) lovastatin [77.2 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) lovastatin [154.5 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) miconazole [0.025 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) miconazole [0.05 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) miconazole [0.1 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) miconazole [0.2 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) fluconazole [16.3 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) fluconazole [32.6 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) fluconazole [65.3 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) fluconazole [130.6 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) fenpropimorph [1.2 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) fenpropimorph [2.3 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) fenpropimorph [4.7 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) alverine citrate [500 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) dyclonine [250 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative
Giaever G~Davis RW, 2004 growth (relative abundance in pooled culture) dyclonine [500 uM], time [o/n + 20 gen] YPD het 5,722 Quantitative

Curation history

Data

Dec. 13, 2016 Ready to load.
April 15, 2017 Loaded.

Tested strains

Dec. 13, 2016 Ready to load.
April 15, 2017 Loaded.