Page N~Bussey H, 2003

Pubmed ID 12663529
Title A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin.
Authors Nicolas Pagé, Manon Gérard-Vincent, Patrice Ménard, Maude Beaulieu, Masayuki Azuma, Gerrit J P Dijkgraaf, Huijuan Li, José Marcoux, Thuy Nguyen, Tim Dowse, Anne-Marie Sdicu, Howard Bussey
Abstract Using the set of Saccharomyces cerevisiae mutants individually deleted for 5718 yeast genes, we screened for altered sensitivity to the antifungal protein, K1 killer toxin, that binds to a cell wall beta-glucan receptor and subsequently forms lethal pores in the plasma membrane. Mutations in 268 genes, including 42 in genes of unknown function, had a phenotype, often mild, with 186 showing resistance and 82 hypersensitivity compared to wild type. Only 15 of these genes were previously known to cause a toxin phenotype when mutated. Mutants for 144 genes were analyzed for alkali-soluble beta-glucan levels; 63 showed alterations. Further, mutants for 118 genes with altered toxin sensitivity were screened for SDS, hygromycin B, and calcofluor white sensitivity as indicators of cell surface defects; 88 showed some additional defect. There is a markedly nonrandom functional distribution of the mutants. Many genes affect specific areas of cellular activity, including cell wall glucan and mannoprotein synthesis, secretory pathway trafficking, lipid and sterol biosynthesis, and cell surface signal transduction, and offer new insights into these processes and their integration.
Citation Genetics 2003; 163:875-94

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Page N~Bussey H, 2003 growth (area of killing zone) K1 killer toxin [unknown] hap alpha N/A Quantitative only for hits
Page N~Bussey H, 2003 growth (area of killing zone) K1 killer toxin [unknown] hom N/A Quantitative only for hits
Page N~Bussey H, 2003 growth (area of killing zone) K1 killer toxin [unknown] het N/A Quantitative only for hits

Curation history

Data

March 27, 2014 Not available.
June 22, 2016 Ready to load.
June 30, 2016 Loaded.

Tested strains

March 27, 2014 Not available.