D'Elia R~Hammond TG, 2005

Pubmed ID 15968571
Title Homozygous diploid deletion strains of Saccharomyces cerevisiae that determine lag phase and dehydration tolerance.
Authors Riccardo D'Elia, Patricia L Allen, Kelly Johanson, Cheryl A Nickerson, Timothy G Hammond
Abstract This study identifies genes that determine length of lag phase, using the model eukaryotic organism, Saccharomyces cerevisiae. We report growth of a yeast deletion series following variations in the lag phase induced by variable storage times after drying-down yeast on filters. Using a homozygous diploid deletion pool, lag times ranging from 0 h to 90 h were associated with increased drop-out of mitochondrial genes and increased survival of nuclear genes. Simple linear regression (R2 analysis) shows that there are over 500 genes for which > 70% of the variation can be explained by lag alone. In the genes with a positive correlation, such that the gene abundance increases with lag and hence the deletion strain is suitable for survival during prolonged storage, there is a strong predominance of nucleonic genes. In the genes with a negative correlation, such that the gene abundance decreases with lag and hence the strain may be critical for getting yeast out of the lag phase, there is a strong predominance of glycoproteins and transmembrane proteins. This study identifies yeast deletion strains with survival advantage on prolonged storage and amplifies our understanding of the genes critical for getting out of the lag phase.
Citation Appl. Microbiol. Biotechnol. 2005; 67:816-26

Datasets

Download the list of datasets
Paper Phenotype Condition Reference Collection Tested mutants Data Details
D'Elia R~Hammond TG, 2005 response to dehydration (correlation between dehydration time and lag phase) YPD [standard] hom ~4,300 Quantitative only for hits
D'Elia R~Hammond TG, 2005 growth (relative abundance in pooled culture) dessication [6 months] hom ~4,300 Quantitative only for hits

Curation history

Tested strains

Aug. 28, 2016 Not available.

Data

Oct. 17, 2014 To request.
June 22, 2016 Request sent.
Aug. 16, 2016 Request sent.
Aug. 28, 2016 Not available.
Aug. 28, 2016 Ready to load.
Sept. 29, 2016 Loaded.