Brown JA~Brown JM, 2006

Pubmed ID 16738548
Title Global analysis of gene function in yeast by quantitative phenotypic profiling.
Authors James A Brown, Gavin Sherlock, Chad L Myers, Nicola M Burrows, Changchun Deng, H Irene Wu, Kelly E McCann, Olga G Troyanskaya, J Martin Brown
Abstract We present a method for the global analysis of the function of genes in budding yeast based on hierarchical clustering of the quantitative sensitivity profiles of the 4756 strains with individual homozygous deletion of nonessential genes to a broad range of cytotoxic or cytostatic agents. This method is superior to other global methods of identifying the function of genes involved in the various DNA repair and damage checkpoint pathways as well as other interrogated functions. Analysis of the phenotypic profiles of the 51 diverse treatments places a total of 860 genes of unknown function in clusters with genes of known function. We demonstrate that this can not only identify the function of unknown genes but can also suggest the mechanism of action of the agents used. This method will be useful when used alone and in conjunction with other global approaches to identify gene function in yeast.
Citation Mol. Syst. Biol. 2006; 2:2006.0001

Datasets

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Paper Phenotype Condition Reference Collection Tested mutants Data Details
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) antimycin a [1 ug/ml], hydrogen peroxide [1 mM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) actinomycin D [400 uM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) antimycin a [1 ug/ml] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) arabinofuranosyl cytidine [400 uM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) arsenic [20-100 uM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) benomyl [10-15 uM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) bleomycin [0.01 U/ml] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) caffeine [6 mM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) calcofluor white [3 ug/ml] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) camptothecin [250-300 uM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) camptothecin [5 ug/ml] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) doxorubicin [0.2 mM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) hydrogen peroxide [3 mM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) hydroxyurea [100 mM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) hygromycin B [7 ug/ml] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) idarubicin [50-100 uM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) lovastatin [100 ug/ml], ethanol [75%] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) mechlorethamine [20 uM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) melphalan [800 uM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) methylmethane sulphonate [0.03%] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) antimycin a [1 ug/ml], raffinose [6%] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) topotecan [20 uM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) tirapazamine [250-300 uM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) UVA [36-288 J/cm2] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) wortmannin [1.5 uM] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) YPD [standard] hom 4,283 Quantitative
Brown JA~Brown JM, 2006 growth (relative abundance in pooled culture) GLYE [standard] hom 4,283 Quantitative

Curation history

Tested strains

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Data

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