Chan TF~Zheng XF, 2000

Pubmed ID 11078525
Title A chemical genomics approach toward understanding the global functions of the target of rapamycin protein (TOR).
Authors T F Chan, J Carvalho, L Riles, X F Zheng
Abstract The target of rapamycin protein (TOR) is a highly conserved ataxia telangiectasia-related protein kinase essential for cell growth. Emerging evidence indicates that TOR signaling is highly complex and is involved in a variety of cellular processes. To understand its general functions, we took a chemical genomics approach to explore the genetic interaction between TOR and other yeast genes on a genomic scale. In this study, the rapamycin sensitivity of individual deletion mutants generated by the Saccharomyces Genome Deletion Project was systematically measured. Our results provide a global view of the rapamycin-sensitive functions of TOR. In contrast to conventional genetic analysis, this approach offers a simple and thorough analysis of genetic interaction on a genomic scale and measures genetic interaction at different possible levels. It can be used to study the functions of other drug targets and to identify novel protein components of a conserved core biological process such as DNA damage checkpoint/repair that is interfered with by a cell-permeable chemical compound.
Citation Proc. Natl. Acad. Sci. U.S.A. 2000; 97:13227-32

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Chan TF~Zheng XF, 2000 growth (streaks on agar) rapamycin [25 nM] hap a ~2,216 Discrete

Curation history

Jan. 31, 2014 Tested strains to request.
Feb. 4, 2014 Data waiting for tested.
March 31, 2014 Tested strains requested.
May 13, 2014 Tested strains requested.
Nov. 11, 2014 Tested strains abandoned.
Nov. 11, 2014 Data to load.
Dec. 3, 2015 Data loaded.