Warringer J~Blomberg A, 2003

Pubmed ID 14676322
Title High-resolution yeast phenomics resolves different physiological features in the saline response.
Authors Jonas Warringer, Elke Ericson, Luciano Fernandez, Olle Nerman, Anders Blomberg
Abstract We present a methodology for gene functional prediction based on extraction of physiologically relevant growth variables from all viable haploid yeast knockout mutants. This quantitative phenomics approach, here applied to saline cultivation, identified marginal but functionally important phenotypes and allowed the precise determination of time to adapt to an environmental challenge, rate of growth, and efficiency of growth. We identified approximately 500 salt-sensitive gene deletions, the majority of which were previously uncharacterized and displayed salt sensitivity for only one of the three physiological features. We also report a high correlation to protein-protein interaction data; in particular, several salt-sensitive subcellular networks indicating functional modules were revealed. In contrast, no correlation was found between gene dispensability and gene expression. It is proposed that high-resolution phenomics will be instrumental in systemwide descriptions of intragenomic functional networks.
Citation Proc. Natl. Acad. Sci. U.S.A. 2003; 100:15724-9


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Papers Phenotype Conditions Collection Tested mutants Data Details
Warringer J~Blomberg A, 2003 growth (exponential growth rate) sodium chloride [0.85 M] hap a 4,702 Quantitative
Warringer J~Blomberg A, 2003 growth (lag phase) sodium chloride [0.85 M] hap a 4,702 Quantitative
Warringer J~Blomberg A, 2003 growth (stationary phase cell density) sodium chloride [0.85 M] hap a 4,700 Quantitative

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March 5, 2013 Data loaded.
March 5, 2013 Tested strains loaded.