North M~Vulpe CD, 2011

Pubmed ID 21912624
Title Genome-wide functional profiling reveals genes required for tolerance to benzene metabolites in yeast.
Authors Matthew North, Vickram J Tandon, Reuben Thomas, Alex Loguinov, Inna Gerlovina, Alan E Hubbard, Luoping Zhang, Martyn T Smith, Chris D Vulpe
Abstract Benzene is a ubiquitous environmental contaminant and is widely used in industry. Exposure to benzene causes a number of serious health problems, including blood disorders and leukemia. Benzene undergoes complex metabolism in humans, making mechanistic determination of benzene toxicity difficult. We used a functional genomics approach to identify the genes that modulate the cellular toxicity of three of the phenolic metabolites of benzene, hydroquinone (HQ), catechol (CAT) and 1,2,4-benzenetriol (BT), in the model eukaryote Saccharomyces cerevisiae. Benzene metabolites generate oxidative and cytoskeletal stress, and tolerance requires correct regulation of iron homeostasis and the vacuolar ATPase. We have identified a conserved bZIP transcription factor, Yap3p, as important for a HQ-specific response pathway, as well as two genes that encode putative NAD(P)H:quinone oxidoreductases, PST2 and YCP4. Many of the yeast genes identified have human orthologs that may modulate human benzene toxicity in a similar manner and could play a role in benzene exposure-related disease.
Citation PLoS ONE 2011; 6:e24205

Datasets

Download the list of datasets
Paper Phenotype Condition Reference Collection Tested mutants Data Details
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) time [5 generations], hydroquinone [1 mM] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) hydroquinone [1 mM], time [15 generations] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) hydroquinone [2 mM], time [5 generations] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) hydroquinone [4 mM], time [5 generations] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) time [15 generations], hydroquinone [2 mM] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) hydroquinone [4 mM], time [15 generations] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) catechol [0.55 mM], time [5 generations] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) time [5 generations], catechol [1.1 mM] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) time [5 generations], catechol [2.2 mM] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) time [15 generations], catechol [0.55 mM] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) catechol [1.1 mM], time [15 generations] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) time [15 generations], catechol [2.2 mM] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) 1,2,4-benzenetriol [87.5 uM], time [5 generations] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) 1,2,4-benzenetriol [350 uM], time [5 generations] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) time [5 generations], 1,2,4-benzenetriol [175 uM] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) time [15 generations], 1,2,4-benzenetriol [87.5 uM] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) time [15 generations], 1,2,4-benzenetriol [350 uM] hom ~4,607 Quantitative only for hits
North M~Vulpe CD, 2011 growth (relative abundance in pooled culture) time [15 generations], 1,2,4-benzenetriol [175 uM] hom ~4,607 Quantitative only for hits

Curation history

Tested strains

June 10, 2016 To request.
June 10, 2016 Request sent.
Aug. 17, 2016 Request sent.
April 25, 2017 Request abandoned.

Data

June 10, 2016 To request.
June 10, 2016 Request sent.
Aug. 17, 2016 Request sent.
April 25, 2017 Request abandoned.
April 25, 2017 Ready to load.
May 8, 2017 Loaded.