Kubota S~Hirata D, 2004

Pubmed ID 15118337
Title Effect of ethanol on cell growth of budding yeast: genes that are important for cell growth in the presence of ethanol.
Authors Shunsuke Kubota, Ikuko Takeo, Kazunori Kume, Muneyoshi Kanai, Atsunori Shitamukai, Masaki Mizunuma, Tokichi Miyakawa, Hitoshi Shimoi, Haruyuki Iefuji, Dai Hirata
Abstract The budding yeast Saccharomyces cerevisiae has been used in the fermentation of various kinds of alcoholic beverages. But the effect of ethanol on the cell growth of this yeast is poorly understood. This study shows that the addition of ethanol causes a cell-cycle delay associated with a transient dispersion of F-actin cytoskeleton, resulting in an increase in cell size. We found that the tyrosine kinase Swe1, the negative regulator of Cdc28-Clb kinase, is related to the regulation of cell growth in the presence of ethanol. Indeed, the increase in cell size due to ethanol was partially abolished in the SWE1-deleted cells, and the amount of Swe1 protein increased transiently in the presence of ethanol. These results indicated that Swe1 is involved in cell size control in the presence of ethanol, and that a signal produced by ethanol causes a transient up-regulation of Swe1. Further we investigated comprehensively the ethanol-sensitive strains in the complete set of 4847 non-essential gene deletions and identified at least 256 genes that are important for cell growth in the presence of ethanol.
Citation Biosci Biotechnol Biochem 2004; 68:968-72
Data abstract Deletion collection was tested for growth in the presence of ethanol.


Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Kubota S~Hirata D, 2004 growth (undefined) ethanol [11%] YPD hap a ~4,847 Discrete

Curation history

Tested strains

Feb. 4, 2014 To request.
March 31, 2014 Request sent.
Nov. 11, 2014 Request sent.
Jan. 28, 2015 Request abandoned.


Feb. 4, 2014 Waiting for tested.
Jan. 28, 2015 Ready to load.
July 11, 2016 Loaded.