Deutschbauer AM~Giaever G, 2005

Pubmed ID 15716499
Title Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast.
Authors Adam M Deutschbauer, Daniel F Jaramillo, Michael Proctor, Jochen Kumm, Maureen E Hillenmeyer, Ronald W Davis, Corey Nislow, Guri Giaever
Abstract Haploinsufficiency is defined as a dominant phenotype in diploid organisms that are heterozygous for a loss-of-function allele. Despite its relevance to human disease, neither the extent of haploinsufficiency nor its precise molecular mechanisms are well understood. We used the complete set of Saccharomyces cerevisiae heterozygous deletion strains to survey the genome for haploinsufficiency via fitness profiling in rich (YPD) and minimal media to identify all genes that confer a haploinsufficient growth defect. This assay revealed that approximately 3% of all approximately 5900 genes tested are haploinsufficient for growth in YPD. This class of genes is functionally enriched for metabolic processes carried out by molecular complexes such as the ribosome. Much of the haploinsufficiency in YPD is alleviated by slowing the growth rate of each strain in minimal media, suggesting that certain gene products are rate limiting for growth only in YPD. Overall, our results suggest that the primary mechanism of haploinsufficiency in yeast is due to insufficient protein production. We discuss the relevance of our findings in yeast to human haploinsufficiency disorders.
Citation Genetics 2005; 169:1915-25

Datasets

Download the list of datasets
Papers Phenotype Conditions Collection Tested mutants Data Details
Deutschbauer AM~Giaever G, 2005 growth (relative abundance in pooled culture) YPD [standard] het 5,773 Quantitative
Deutschbauer AM~Giaever G, 2005 growth (relative abundance in pooled culture) minimal complete [standard] het 5,668 Quantitative
Deutschbauer AM~Giaever G, 2005 growth (relative abundance in pooled culture) YPD [standard] hom 4,731 Quantitative
Deutschbauer AM~Giaever G, 2005 growth (relative abundance in pooled culture) minimal complete [standard] hom 4,670 Quantitative

Curation history

Nov. 1, 2016 Tested strains to load.
Nov. 1, 2016 Data to load.
Jan. 29, 2017 Tested strains loaded.
Jan. 29, 2017 Data loaded.