Lesuisse E~Dancis A, 2005

Pubmed ID 15489514
Title Genome-wide screen for genes with effects on distinct iron uptake activities in Saccharomyces cerevisiae.
Authors Emmanuel Lesuisse, Simon A B Knight, Maïté Courel, Renata Santos, Jean-Michel Camadro, Andrew Dancis
Abstract We screened a collection of 4847 haploid knockout strains (EUROSCARF collection) of Saccharomyces cerevisiae for iron uptake from the siderophore ferrioxamine B (FOB). A large number of mutants showed altered uptake activities, and a few turned yellow when grown on agar plates with added FOB, indicating increased intracellular accumulation of undissociated siderophores. A subset consisting of 197 knockouts with altered uptake was examined further for regulated activities that mediate cellular uptake of iron from other siderophores or from iron salts. Hierarchical clustering analysis grouped the data according to iron sources and according to mutant categories. In the first analysis, siderophores grouped together with the exception of enterobactin, which grouped with iron salts, suggesting a reductive pathway of iron uptake for this siderophore. Mutant groupings included three categories: (i) high-FOB uptake, high reductase, low-ferrous transport; (ii) isolated high- or low-FOB transport; and (iii) induction of all activities. Mutants with statistically altered uptake activities included genes encoding proteins with predominant localization in the secretory pathway, nucleus, and mitochondria. Measurements of different iron-uptake activities in the yeast knockout collection make possible distinctions between genes with general effects on iron metabolism and those with pathway-specific effects.
Citation Genetics 2005; 169:107-22

Datasets

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Paper Phenotype Condition Reference Collection Tested mutants Data Details
Lesuisse E~Dancis A, 2005 siderophore uptake (ferrioxamine B (FOB)) standard hap a 4,652 Quantitative
Lesuisse E~Dancis A, 2005 ferrireductase activity standard hap a 4,700 Quantitative
Lesuisse E~Dancis A, 2005 ferrous uptake (ferrous ascorbate (F(II))) standard hap a 4,701 Quantitative

Curation history

Tested strains

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Data

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