Pereira FB~Domingues L, 2014

Pubmed ID 25287021
Title Genome-wide screening of Saccharomyces cerevisiae genes required to foster tolerance towards industrial wheat straw hydrolysates.
Authors Francisco B Pereira, Miguel C Teixeira, Nuno P Mira, Isabel Sá-Correia, Lucília Domingues
Abstract The presence of toxic compounds derived from biomass pre-treatment in fermentation media represents an important drawback in second-generation bio-ethanol production technology and overcoming this inhibitory effect is one of the fundamental challenges to its industrial production. The aim of this study was to systematically identify, in industrial medium and at a genomic scale, the Saccharomyces cerevisiae genes required for simultaneous and maximal tolerance to key inhibitors of lignocellulosic fermentations. Based on the screening of EUROSCARF haploid mutant collection, 242 and 216 determinants of tolerance to inhibitory compounds present in industrial wheat straw hydrolysate (WSH) and in inhibitor-supplemented synthetic hydrolysate were identified, respectively. Genes associated to vitamin metabolism, mitochondrial and peroxisomal functions, ribosome biogenesis and microtubule biogenesis and dynamics are among the newly found determinants of WSH resistance. Moreover, PRS3, VMA8, ERG2, RAV1 and RPB4 were confirmed as key genes on yeast tolerance and fermentation of industrial WSH.
Citation J. Ind. Microbiol. Biotechnol. 2014; 41:1753-61


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Papers Phenotype Conditions Collection Tested mutants Data Details
Pereira FB~Domingues L, 2014 growth (spot assay) wheat straw hydrolysate [unknown] hap alpha 4,742 Discrete
Pereira FB~Domingues L, 2014 growth (spot assay) synthetic hydrolysate [unknown] hap alpha 4,742 Discrete

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