Zhao R~Houry WA, 2005

Pubmed ID 15766533
Title Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone.
Authors Rongmin Zhao, Mike Davey, Ya-Chieh Hsu, Pia Kaplanek, Amy Tong, Ainslie B Parsons, Nevan Krogan, Gerard Cagney, Duy Mai, Jack Greenblatt, Charles Boone, Andrew Emili, Walid A Houry
Abstract Physical, genetic, and chemical-genetic interactions centered on the conserved chaperone Hsp90 were mapped at high resolution in yeast using systematic proteomic and genomic methods. Physical interactions were identified using genome-wide two hybrid screens combined with large-scale affinity purification of Hsp90-containing protein complexes. Genetic interactions were uncovered using synthetic genetic array technology and by a microarray-based chemical-genetic screen of a set of about 4700 viable yeast gene deletion mutants for hypersensitivity to the Hsp90 inhibitor geldanamycin. An extended network, consisting of 198 putative physical interactions and 451 putative genetic and chemical-genetic interactions, was found to connect Hsp90 to cofactors and substrates involved in a wide range of cellular functions. Two novel Hsp90 cofactors, Tah1 (YCR060W) and Pih1 (YHR034C), were also identified. These cofactors interact physically and functionally with the conserved AAA(+)-type DNA helicases Rvb1/Rvb2, which are key components of several chromatin remodeling factors, thereby linking Hsp90 to epigenetic gene regulation.
Citation Cell 2005; 120:715-27

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Zhao R~Houry WA, 2005 growth (relative abundance in pooled culture) geldanamycin [20 ug/ml] hap a ~4,800 Quantitative only for hits

Curation history

Nov. 14, 2014 Tested strains to request.
Nov. 14, 2014 Data to request.
Nov. 19, 2014 Tested strains requested.
Nov. 19, 2014 Data requested.
Nov. 24, 2014 Data not available.
Nov. 24, 2014 Data waiting for tested.
Nov. 25, 2014 Tested strains not available.
Nov. 25, 2014 Data to load.
Oct. 19, 2016 Data loaded.