Schneider J~Shilatifard A, 2005

Pubmed ID 16168379
Title Molecular regulation of histone H3 trimethylation by COMPASS and the regulation of gene expression.
Authors Jessica Schneider, Adam Wood, Jung-Shin Lee, Rebecca Schuster, Jeff Dueker, Courtney Maguire, Selene K Swanson, Laurence Florens, Michael P Washburn, Ali Shilatifard
Abstract The Set1-containing complex COMPASS, which is the yeast homolog of the human MLL complex, is required for mono-, di-, and trimethylation of lysine 4 of histone H3. We have performed a comparative global proteomic screen to better define the role of COMPASS in histone trimethylation. We report that both Cps60 and Cps40 components of COMPASS are required for proper histone H3 trimethylation, but not for proper regulation of telomere-associated gene silencing. Purified COMPASS lacking Cps60 can mono- and dimethylate but is not capable of trimethylating H3(K4). Chromatin immunoprecipitation (ChIP) studies indicate that the loss subunits of COMPASS required for histone trimethylation do not affect the localization of Set1 to chromatin for the genes tested. Collectively, our results suggest a molecular requirement for several components of COMPASS for proper histone H3 trimethylation and regulation of telomere-associated gene expression, indicating multiple roles for different forms of histone methylation by COMPASS.
Citation Mol. Cell 2005; 19:849-56

Datasets

Download the list of datasets
Papers Phenotype Conditions Collection Tested mutants Data Details
Schneider J~Shilatifard A, 2005 protein/peptide methylation (H3K79) standard [standard] hap a N/A None
Schneider J~Shilatifard A, 2005 protein/peptide methylation (H3K79) standard [standard] hap alpha N/A None

Curation history

Sept. 22, 2016 Tested strains abandoned.
Sept. 22, 2016 Data abandoned.