Pan X~Boeke JD, 2004

Pubmed ID 15525520
Title A robust toolkit for functional profiling of the yeast genome.
Authors Xuewen Pan, Daniel S Yuan, Dong Xiang, Xiaoling Wang, Sharon Sookhai-Mahadeo, Joel S Bader, Philip Hieter, Forrest Spencer, Jef D Boeke
Abstract Study of mutant phenotypes is a fundamental method for understanding gene function. The construction of a near-complete collection of yeast knockouts (YKO) and the unique molecular barcodes (or TAGs) that identify each strain has enabled quantitative functional profiling of Saccharomyces cerevisiae. By using these TAGs and the SGA reporter, MFA1pr-HIS3, which facilitates conversion of heterozygous diploid YKO strains into haploid mutants, we have developed a set of highly efficient microarray-based techniques, collectively referred as dSLAM (diploid-based synthetic lethality analysis on microarrays), to probe genome-wide gene-chemical and gene-gene interactions. Direct comparison revealed that these techniques are more robust than existing methods in functional profiling of the yeast genome. Widespread application of these tools will elucidate a comprehensive yeast genetic network.
Citation Mol. Cell 2004; 16:487-96

Datasets

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Paper Phenotype Condition Reference Collection Tested mutants Data Details
Pan X~Boeke JD, 2004 growth (relative abundance in pooled culture) benomyl [1 ug/ml] hap a 4,490 Quantitative only for hits
Pan X~Boeke JD, 2004 growth (relative abundance in pooled culture) benomyl [5 ug/ml] hap a 4,490 Quantitative only for hits
Pan X~Boeke JD, 2004 growth (relative abundance in pooled culture) benomyl [10 ug/ml] hap a 4,490 Quantitative only for hits
Pan X~Boeke JD, 2004 growth (relative abundance in pooled culture) benomyl [15 ug/ml] hap a 4,490 Quantitative only for hits
Pan X~Boeke JD, 2004 growth (relative abundance in pooled culture) benomyl [20 ug/ml] hap a 4,490 Quantitative only for hits
Pan X~Boeke JD, 2004 growth (relative abundance in pooled culture) benomyl [25 ug/ml] hap a 4,490 Quantitative only for hits
Pan X~Boeke JD, 2004 growth (relative abundance in pooled culture) benomyl [30 ug/ml] hap a 4,490 Quantitative only for hits
Pan X~Boeke JD, 2004 growth (relative abundance in pooled culture) benomyl [35 ug/ml] hap a 4,490 Quantitative only for hits
Pan X~Boeke JD, 2004 growth (relative abundance in pooled culture) benomyl [40 ug/ml] hap a 4,490 Quantitative only for hits

Curation history

Tested strains

Oct. 26, 2016 Ready to load.
April 14, 2017 Loaded.

Data

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April 14, 2017 Loaded.