Rand JD~Grant CM, 2006

Pubmed ID 16251355
Title The thioredoxin system protects ribosomes against stress-induced aggregation.
Authors Jonathan D Rand, Chris M Grant
Abstract We previously showed that thioredoxins are required for dithiothreitol (DTT) tolerance, suggesting they maintain redox homeostasis in response to both oxidative and reductive stress conditions. In this present study, we screened the complete set of viable deletion strains in Saccharomyces cerevisiae for sensitivity to DTT to identify cell functions involved in resistance to reductive stress. We identified 195 mutants, whose gene products are localized throughout the cell. DTT-sensitive mutants were distributed among most major biological processes, but they particularly affected gene expression, metabolism, and the secretory pathway. Strikingly, a mutant lacking TSA1, encoding a peroxiredoxin, showed a similar sensitivity to DTT as a thioredoxin mutant. Epistasis analysis indicated that thioredoxins function upstream of Tsa1 in providing tolerance to DTT. Our data show that the chaperone function of Tsa1, rather than its peroxidase function, is required for this activity. Cells lacking TSA1 were found to accumulate aggregated proteins, and this was exacerbated by exposure to DTT. Analysis of the protein aggregates revealed that they are predominantly composed of ribosomal proteins. Furthermore, aggregation was found to correlate with an inhibition of translation initiation. We propose that Tsa1 normally functions to chaperone misassembled ribosomal proteins, preventing the toxicity that arises from their aggregation.
Citation Mol. Biol. Cell 2006; 17:387-401

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Rand JD~Grant CM, 2006 growth (spot assay) dithiothreitol [32 mM] hom 4,692 Discrete

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Dec. 1, 2016 Tested strains to request.
Dec. 1, 2016 Data to request.
Feb. 7, 2017 Tested strains requested.
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April 17, 2017 Tested strains loaded.
April 17, 2017 Data loaded.