Chasse SA~Dohlman HG, 2006

Pubmed ID 16467474
Title Genome-scale analysis reveals Sst2 as the principal regulator of mating pheromone signaling in the yeast Saccharomyces cerevisiae.
Authors Scott A Chasse, Paul Flanary, Stephen C Parnell, Nan Hao, Jiyoung Y Cha, David P Siderovski, Henrik G Dohlman
Abstract A common property of G protein-coupled receptors is that they become less responsive with prolonged stimulation. Regulators of G protein signaling (RGS proteins) are well known to accelerate G protein GTPase activity and do so by stabilizing the transition state conformation of the G protein alpha subunit. In the yeast Saccharomyces cerevisiae there are four RGS-homologous proteins (Sst2, Rgs2, Rax1, and Mdm1) and two Galpha proteins (Gpa1 and Gpa2). We show that Sst2 is the only RGS protein that binds selectively to the transition state conformation of Gpa1. The other RGS proteins also bind Gpa1 and modulate pheromone signaling, but to a lesser extent and in a manner clearly distinct from Sst2. To identify other candidate pathway regulators, we compared pheromone responses in 4,349 gene deletion mutants representing nearly all nonessential genes in yeast. A number of mutants produced an increase (sst2, bar1, asc1, and ygl024w) or decrease (cla4) in pheromone sensitivity or resulted in pheromone-independent signaling (sst2, pbs2, gas1, and ygl024w). These findings suggest that Sst2 is the principal regulator of Gpa1-mediated signaling in vivo but that other proteins also contribute in distinct ways to pathway regulation.
Citation Eukaryotic Cell 2006; 5:330-46

Datasets

Download the list of datasets
Papers Phenotype Conditions Collection Tested mutants Data Details
Chasse SA~Dohlman HG, 2006 pheromone sensitivity (FUS1-lacZ basal activity) alpha factor pheromone [0.3-100 μM] hap a 4,760 Discrete
Chasse SA~Dohlman HG, 2006 pheromone sensitivity (FUS1-lacZ EC50) alpha factor pheromone [0.3-100 μM] hap a 4,760 Discrete
Chasse SA~Dohlman HG, 2006 pheromone sensitivity (FUS1-lacZ maximum responsiveness) alpha factor pheromone [0.3-100 μM] hap a 4,760 Discrete

Curation history

Oct. 17, 2016 Tested strains to request.
Oct. 17, 2016 Data to request.
April 28, 2017 Tested strains requested.
April 28, 2017 Data requested.
May 9, 2017 Tested strains to load.
May 9, 2017 Data to load.
June 15, 2017 Tested strains loaded.
June 15, 2017 Data loaded.