Slessareva JE~Dohlman HG, 2006

Pubmed ID 16839886
Title Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome.
Authors Janna E Slessareva, Sheri M Routt, Brenda Temple, Vytas A Bankaitis, Henrik G Dohlman
Abstract In the yeast Saccharomyces cerevisiae, the G protein beta gamma subunits are essential for pheromone signaling. The Galpha subunit Gpa1 can also promote signaling, but the effectors in this pathway are not well characterized. To identify candidate Gpa1 effectors, we expressed the constitutively active Gpa1(Q323L) mutant in each of nearly 5000 gene-deletion strains and measured mating-specific responses. Our analysis reveals a requirement for both the catalytic (Vps34) and regulatory (Vps15) subunits of the sole phosphatidylinositol 3-kinase in yeast. We demonstrate that Gpa1 is present at endosomes, where it interacts directly with both Vps34 and Vps15 and stimulates increased production of phosphatidylinositol 3-phosphate. Notably, Vps15 binds to GDP-bound Gpa1 and is predicted to have a seven-WD repeat structure similar to that of known G protein beta subunits. These findings reveal two new components of the pheromone signaling pathway. More remarkably, these proteins appear to comprise a preformed effector-G beta subunit assembly and function at the endosome rather than at the plasma membrane.
Citation Cell 2006; 126:191-203


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Slessareva JE~Dohlman HG, 2006 gene expression GPA1-Q323L mutant [24 hours] hap a ~4,847 Discrete

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