Shankarnarayan S~Fassler JS, 2008

Pubmed ID 18048366
Title Modulation of yeast Sln1 kinase activity by the CCW12 cell wall protein.
Authors Sandhya Shankarnarayan, Sandhya S Narang, Cheryl L Malone, Robert J Deschenes, Jan S Fassler
Abstract The yeast Sln1p sensor kinase is best known as an osmosensor involved in the regulation of the hyperosmolarity glycerol mitogen-activated protein kinase cascade. Down-regulation of Sln1 kinase activity occurs under hypertonic conditions and leads to phosphorylation of the Hog1p mitogen-activated protein kinase and increased osmotic stress-response gene expression. Conditions leading to kinase up-regulation include osmotic imbalance caused by glycerol retention in the glycerol channel mutant, fps1 (Tao, W., Deschenes, R. J., and Fassler, J. S. (1999) J. Biol. Chem. 274, 360-367). The hypothesis that Sln1p kinase activity is responsive to turgor was first suggested by the increased Sln1p kinase activity in mutants lacking Fps1p in which glycerol accumulation leads to water uptake. Also consistent with the turgor hypothesis is the observation that reduced turgor caused by treatment of cells with nystatin, a drug that increases membrane permeability and causes cell shrinkage, reduced Sln1p kinase activity (Tao, W., Deschenes, R. J., and Fassler, J. S. (1999) J. Biol. Chem. 274, 360-367; Reiser, V., Raitt, D. C., and Saito, H. (2003) J. Cell Biol. 161, 1035-1040). The turgor hypothesis is revisited here in the context of the identification and characterization of the cell wall gene, CCW12, as a determinant of Sln1p activity. Results of this analysis suggest that the activity of the plasma membrane localized Sln1p is affected by the presence or absence of specific outer cell wall proteins and that this effect is independent of turgor.
Citation J. Biol. Chem. 2008; 283:1962-73


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Papers Phenotype Conditions Collection Tested mutants Data Details
Shankarnarayan S~Fassler JS, 2008 expression level of a gene (OCH1) standard [standard] hap alpha N/A None

Curation history


Dec. 14, 2016 Data clarification needed.
Feb. 1, 2017 Data requested.
June 23, 2017 Data requested.
June 26, 2017 Data not available.

Tested strains

Dec. 14, 2016 Tested strains to request.
Feb. 1, 2017 Tested strains requested.
June 23, 2017 Tested strains requested.
June 26, 2017 Tested strains not available.