Berry DB~Gasch AP, 2011

Pubmed ID 22102822
Title Multiple means to the same end: the genetic basis of acquired stress resistance in yeast.
Authors David B Berry, Qiaoning Guan, James Hose, Suraiya Haroon, Marinella Gebbia, Lawrence E Heisler, Corey Nislow, Guri Giaever, Audrey P Gasch
Abstract In nature, stressful environments often occur in combination or close succession, and thus the ability to prepare for impending stress likely provides a significant fitness advantage. Organisms exposed to a mild dose of stress can become tolerant to what would otherwise be a lethal dose of subsequent stress; however, the mechanism of this acquired stress tolerance is poorly understood. To explore this, we exposed the yeast gene-deletion libraries, which interrogate all essential and non-essential genes, to successive stress treatments and identified genes necessary for acquiring subsequent stress resistance. Cells were exposed to one of three different mild stress pretreatments (salt, DTT, or heat shock) and then challenged with a severe dose of hydrogen peroxide (H(2)O(2)). Surprisingly, there was little overlap in the genes required for acquisition of H(2)O(2) tolerance after different mild-stress pretreatments, revealing distinct mechanisms of surviving H(2)O(2) in each case. Integrative network analysis of these results with respect to protein-protein interactions, synthetic-genetic interactions, and functional annotations identified many processes not previously linked to H(2)O(2) tolerance. We tested and present several models that explain the lack of overlap in genes required for H(2)O(2) tolerance after each of the three pretreatments. Together, this work shows that acquired tolerance to the same severe stress occurs by different mechanisms depending on prior cellular experiences, underscoring the context-dependent nature of stress tolerance.
Citation PLoS Genet. 2011; 7:e1002353

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) hydrogen peroxide [1-1.2 mM], sodium chloride [0.7 M] hom 4,289 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) temperature [40ºC], hydrogen peroxide [1-1.2 mM] hom 4,228 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) dithiothreitol [2.5 mM], hydrogen peroxide [1-1.2 mM] hom 4,242 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) standard [standard] hom 4,564 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) hydrogen peroxide [0.4 mM] hom 4,417 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) sodium chloride [0.7 M] hom 4,297 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) dithiothreitol [2.5 mM] hom 4,274 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) temperature [40ºC] hom 4,303 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) standard [standard] hom 4,455 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) hydrogen peroxide [0.4 mM] hom 4,508 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) dithiothreitol [2.5 mM] hom 4,347 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) sodium chloride [0.7 M] hom 4,448 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) temperature [40ºC] hom 4,314 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) tunicamycin [20 uM] hom 4,329 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) dithiothreitol [2.5 mM], hydrogen peroxide [1-1.2 mM] hom 4,331 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) hydrogen peroxide [1-1.2 mM], sodium chloride [0.7 M] hom 4,405 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) temperature [40ºC], hydrogen peroxide [1-1.2 mM] hom 4,344 Quantitative
Berry DB~Gasch AP, 2011 growth (relative abundance in pooled culture) hydrogen peroxide [1-1.2 mM], tunicamycin [20 uM] hom 4,294 Quantitative

Curation history

Data

Nov. 25, 2014 Data loaded.

Tested strains

Nov. 25, 2014 Tested strains loaded.