Hendry JA~Brown GW, 2015

Pubmed ID 25721128
Title Leveraging DNA damage response signaling to identify yeast genes controlling genome stability.
Authors Jason A Hendry, Guihong Tan, Jiongwen Ou, Charles Boone, Grant W Brown
Abstract Oncogenesis frequently is accompanied by rampant genome instability, which fuels genetic heterogeneity and resistance to targeted cancer therapy. We have developed an approach that allows precise, quantitative measurement of genome instability in high-throughput format in the Saccharomyces cerevisiae model system. Our approach takes advantage of the strongly DNA damage-inducible gene RNR3, in conjunction with the reporter synthetic genetic array methodology, to infer mutants exhibiting genome instability by assaying for increased Rnr3 abundance. We screen for genome instability across a set of ~1000 essential and ~4200 nonessential mutant yeast alleles in untreated conditions and in the presence of the DNA-damaging agent methylmethane sulfonate. Our results provide broad insights into the cellular processes and pathways required for genome maintenance. Through comparison with existing genome instability screens, we isolated 130 genes that had not previously been linked to genome maintenance, 51% of which have human homologs. Several of these homologs are associated with a genome instability phenotype in human cells or are causally mutated in cancer. A comprehensive understanding of the processes required to prevent genome instability will facilitate a better understanding of its sources in oncogenesis.
Citation G3 (Bethesda) 2015; 5:997-1006

Datasets

Download the list of datasets
Paper Phenotype Condition Reference Collection Tested mutants Data Details
Hendry JA~Brown GW, 2015 abundance of a specific protein/peptide (Rnr3) methylmethane sulphonate [0.03%] hap a 4,140 Quantitative
Hendry JA~Brown GW, 2015 abundance of a specific protein/peptide (Rnr3) YPD [standard] hap a 4,146 Quantitative

Curation history

Tested strains

June 2, 2015 Ready to load.
March 3, 2016 Loaded.

Data

June 2, 2015 Ready to load.
March 3, 2016 Loaded.