Jonikas MC~Schuldiner M, 2009

Pubmed ID 19325107
Title Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum.
Authors Martin C Jonikas, Sean R Collins, Vladimir Denic, Eugene Oh, Erin M Quan, Volker Schmid, Jimena Weibezahn, Blanche Schwappach, Peter Walter, Jonathan S Weissman, Maya Schuldiner
Abstract Protein folding in the endoplasmic reticulum is a complex process whose malfunction is implicated in disease and aging. By using the cell's endogenous sensor (the unfolded protein response), we identified several hundred yeast genes with roles in endoplasmic reticulum folding and systematically characterized their functional interdependencies by measuring unfolded protein response levels in double mutants. This strategy revealed multiple conserved factors critical for endoplasmic reticulum folding, including an intimate dependence on the later secretory pathway, a previously uncharacterized six-protein transmembrane complex, and a co-chaperone complex that delivers tail-anchored proteins to their membrane insertion machinery. The use of a quantitative reporter in a comprehensive screen followed by systematic analysis of genetic dependencies should be broadly applicable to functional dissection of complex cellular processes from yeast to human.
Citation Science 2009; 323:1693-7


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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Jonikas MC~Schuldiner M, 2009 protein/peptide metabolism (unfolded protein response (UPR)) standard YPD + G418 [200 ug/ml] hap alpha 4,527 Quantitative

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