Saka K~Kobayashi T, 2016

Pubmed ID 26912831
Title More than 10% of yeast genes are related to genome stability and influence cellular senescence via rDNA maintenance.
Authors Kimiko Saka, Akihiro Takahashi, Mariko Sasaki, Takehiko Kobayashi
Abstract Genome instability triggers cellular senescence and is a common cause of cancer. The ribosomal RNA genes (rDNA), due to their repetitive structure, form a fragile site with frequent rearrangements. To identify eukaryotic factors that connect reduced genome stability to senescence we screened 4,876 strains of a Saccharomyces cerevisiae deletion library for aberrant rDNA and found 708 genes that contribute to its upkeep. 28 mutants caused abnormalities in non-rDNA chromosomes and among them 12 mutants have abnormalities both in rDNA and in non-rDNA chromosomes. Many mutated genes have not previously been implicated with genome maintenance nor their homologues with tumorigenesis in mammals. The link between rDNA state and senescence was broken after deletion of factors related with DNA polymerase ϵ. These mutations also suppressed the short lifespan phenotype of a sir2 mutant, suggesting a model in which molecular events at the heart of the replication fork induce abnormal rDNA recombination and are responsible for the emergence of an aging signal.
Citation Nucleic Acids Res. 2016; 44:4211-21

Datasets

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Paper Phenotype Condition Reference Collection Tested mutants Data Details
Saka K~Kobayashi T, 2016 rDNA copy number standard YPD hap a 4,975 Discrete
Saka K~Kobayashi T, 2016 rDNA stability standard YPD hap a 4,975 Discrete

Curation history

Tested strains

April 4, 2016 Ready to load.
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Data

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May 30, 2016 Loaded.