Stauffer B~Powers T, 2015

Pubmed ID 26466677
Title Target of rapamycin signaling mediates vacuolar fission caused by endoplasmic reticulum stress in Saccharomyces cerevisiae.
Authors Bobbiejane Stauffer, Ted Powers
Abstract The yeast vacuole is equivalent to the mammalian lysosome and, in response to diverse physiological and environmental stimuli, undergoes alterations both in size and number. Here we demonstrate that vacuoles fragment in response to stress within the endoplasmic reticulum (ER) caused by chemical or genetic perturbations. We establish that this response does not involve known signaling pathways linked previously to ER stress but instead requires the rapamycin-sensitive TOR Complex 1 (TORC1), a master regulator of cell growth, together with its downstream effectors, Tap42/Sit4 and Sch9. To identify additional factors required for ER stress-induced vacuolar fragmentation, we conducted a high-throughput, genome-wide visual screen for yeast mutants that are refractory to ER stress-induced changes in vacuolar morphology. We identified several genes shown previously to be required for vacuolar fusion and/or fission, validating the utility of this approach. We also identified a number of new components important for fragmentation, including a set of proteins involved in assembly of the V-ATPase. Remarkably, we find that one of these, Vph2, undergoes a change in intracellular localization in response to ER stress and, moreover, in a manner that requires TORC1 activity. Together these results reveal a new role for TORC1 in the regulation of vacuolar behavior.
Citation Mol. Biol. Cell 2015; 26:4618-30

Datasets

Download the list of datasets
Papers Phenotype Conditions Collection Tested mutants Data Details
Stauffer B~Powers T, 2015 vacuolar morphology FM4-64 [1 uM], tunicamycin [1 ug/ml] hap a 4,760 Discrete

Curation history

Data

April 21, 2016 To request.
April 25, 2016 Request sent.
Aug. 19, 2016 Request sent.
Sept. 21, 2016 Request abandoned.
Sept. 21, 2016 Ready to load.
Oct. 3, 2016 Loaded.

Tested strains

April 1, 2016 To request.
April 25, 2016 Request sent.
Aug. 19, 2016 Request sent.
Sept. 21, 2016 Ready to load.
Oct. 3, 2016 Loaded.