Ooi SL~Boeke JD, 2001

Pubmed ID 11701889
Title A DNA microarray-based genetic screen for nonhomologous end-joining mutants in Saccharomyces cerevisiae.
Authors S L Ooi, D D Shoemaker, J D Boeke
Abstract We describe a microarray-based screen performed by imposing different genetic selections on thousands of yeast mutants in parallel, representing most genes in the yeast genome. The presence or absence of mutants was detected by oligonucleotide arrays that hybridize to 20-nucleotide "barcodes." We used this method to screen for components of the nonhomologous end-joining (NHEJ) pathway. Known components of the pathway were identified, as well as a gene not previously known to be involved in NHEJ, NEJ1. Nej1 protein interacts with the amino terminus of LIF1/XRCC4, a recently recognized "guardian of the genome" against cancer.
Citation Science 2001; 294:2552-6


Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Ooi SL~Boeke JD, 2001 NHEJ standard SC - Ura hap a ~4,647 Discrete
Ooi SL~Boeke JD, 2001 NHEJ standard SC - Ura hom ~3,546 Discrete

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