Jiang L~Zhang Y, 2014

Pubmed ID 25331360
Title Cadmium-induced activation of high osmolarity glycerol pathway through its Sln1 branch is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in budding yeast.
Authors Linghuo Jiang, Chunlei Cao, Lilin Zhang, Wei Lin, Jing Xia, Huihui Xu, Yan Zhang
Abstract Cadmium ions disrupt reactive oxygen species/Ca(2+) homeostasis and subsequently elicit cell death and adaptive signaling cascades in eukaryotic cells. Through a functional genomics approach, we have identified deletion mutants of 106 yeast genes, including three MAP kinase genes (HOG1, SLT2, and KSS1), are sensitive to a sublethal concentration of cadmium, and 64 mutants show elevated intracellular cadmium concentrations upon exposure to cadmium. Hog1 is phosphorylated, reaching a peak 30 min after the cadmium treatment. Both Sln1 and Sho1 upstream branches are involved in the cadmium-induced activation of high osmolarity glycerol (HOG) pathway. Cadmium-induced HOG activation is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in the Sln1 branch.
Citation FEMS Yeast Res. 2014; 14:1263-72

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Jiang L~Zhang Y, 2014 growth (colony size) cadmium dichloride [100 uM] YPD hom 4,742 Discrete

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