Zhao Y~Jiang L, 2013

Pubmed ID 23026396
Title Activation of calcineurin is mainly responsible for the calcium sensitivity of gene deletion mutations in the genome of budding yeast.
Authors Yunying Zhao, Jingcai Du, Gang Zhao, Linghuo Jiang
Abstract Here we have identified 120 gene deletion mutants that are sensitive to 0.4M calcium in Saccharomyces cerevisiae. Twenty-seven of these mutants are of genes involved in the vacuolar protein sorting pathway, including those encoding the seven components of the ESCRT complexes, and ten of them encode the components and assembly factors of the vacuolar H(+)-ATPase. Both Mediator and Paf1 complexes modulating the activity of the general transcription machinery are involved in the calcium sensitivity of yeast cells. Most of these mutants show elevated intracellular calcium contents in response to calcium stress. The calcium sensitivity of 106 mutants can be completely suppressed by 10mM Mg(2+), 56 of which can also be suppressed by the inhibitor of calcineurin, cyclosporine A. Therefore, the calcium sensitivity of nearly a half of these 120 mutations is at least partially due to the activation of calcineurin and can be modulated by magnesium ion.
Citation Genomics 2013; 101:49-56

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Zhao Y~Jiang L, 2013 growth (colony size) calcium dichloride [0.4 M] hom 4,743 Discrete

Curation history

Data

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