Stepchenkova EI~Pavlov YI, 2005

Pubmed ID 15932646
Title Genome-wide screening for genes whose deletions confer sensitivity to mutagenic purine base analogs in yeast.
Authors Elena I Stepchenkova, Stanislav G Kozmin, Vladimir V Alenin, Youri I Pavlov
Abstract N-hydroxylated base analogs, such as 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA), are strong mutagens in various organisms due to their ambiguous base-pairing properties. The systems protecting cells from HAP and related noncanonical purines in Escherichia coli include specialized deoxyribonucleoside triphosphatase RdgB, DNA repair endonuclease V, and a molybdenum cofactor-dependent system. Fewer HAP-detoxification systems have been identified in yeast Saccharomyces cerevisiae and other eukaryotes. Cellular systems protecting from AHA are unknown. In the present study, we performed a genome-wide search for genes whose deletions confer sensitivity to HAP and AHA in yeast.
Citation BMC Genet. 2005; 6:31

Datasets

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Paper Phenotype Condition Medium Collection Tested mutants Data Details
Stepchenkova EI~Pavlov YI, 2005 growth (CFU) N(6)-hydroxyadenine [1-100 ug/ml] hap alpha 4,792 Quantitative only for hits
Stepchenkova EI~Pavlov YI, 2005 mutation frequency N(6)-hydroxyadenine [1-100 ug/ml] hap alpha 4,792 Quantitative only for hits

Curation history

Data

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