Stepchenkova EI~Pavlov YI, 2005

Pubmed ID 15932646
Title Genome-wide screening for genes whose deletions confer sensitivity to mutagenic purine base analogs in yeast.
Authors Elena I Stepchenkova, Stanislav G Kozmin, Vladimir V Alenin, Youri I Pavlov
Abstract N-hydroxylated base analogs, such as 6-hydroxylaminopurine (HAP) and 2-amino-6-hydroxylaminopurine (AHA), are strong mutagens in various organisms due to their ambiguous base-pairing properties. The systems protecting cells from HAP and related noncanonical purines in Escherichia coli include specialized deoxyribonucleoside triphosphatase RdgB, DNA repair endonuclease V, and a molybdenum cofactor-dependent system. Fewer HAP-detoxification systems have been identified in yeast Saccharomyces cerevisiae and other eukaryotes. Cellular systems protecting from AHA are unknown. In the present study, we performed a genome-wide search for genes whose deletions confer sensitivity to HAP and AHA in yeast.
Citation BMC Genet. 2005; 6:31

Datasets

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Papers Phenotype Conditions Collection Tested mutants Data Details
Stepchenkova EI~Pavlov YI, 2005 growth (CFU) 6-hydroxylaminopurine [1-100 ug/ml] hap alpha 4,792 Quantitative only for hits
Stepchenkova EI~Pavlov YI, 2005 mutation frequency 6-hydroxylaminopurine [1-100 ug/ml] hap alpha 4,792 Quantitative only for hits

Curation history

April 10, 2013 Data requested.
April 12, 2013 Tested strains requested.
April 12, 2013 Data not available.
April 12, 2013 Data waiting for tested.
April 14, 2013 Tested strains to load.
April 14, 2013 Data to load.
March 10, 2014 Tested strains loaded.
March 10, 2014 Data loaded.