Wu J~Hopper AK, 2015

Pubmed ID 26680305
Title Genome-wide screen uncovers novel pathways for tRNA processing and nuclear-cytoplasmic dynamics.
Authors Jingyan Wu, Alicia Bao, Kunal Chatterjee, Yao Wan, Anita K Hopper
Abstract Transfer ribonucleic acids (tRNAs) are essential for protein synthesis. However, key gene products involved in tRNA biogenesis and subcellular movement remain to be discovered. We conducted the first comprehensive unbiased analysis of the role of nearly an entire proteome in tRNA biology and describe 162 novel and 12 previously known Saccharomyces cerevisiae gene products that function in tRNA processing, turnover, and subcellular movement. tRNA nuclear export is of particular interest because it is essential, but the known tRNA exporters (Los1 [exportin-t] and Msn5 [exportin-5]) are unessential. We report that mutations of CRM1 (Exportin-1), MEX67/MTR2 (TAP/p15), and five nucleoporins cause accumulation of unspliced tRNA, a hallmark of defective tRNA nuclear export. CRM1 mutation genetically interacts with los1Δ and causes altered tRNA nuclear-cytoplasmic distribution. The data implicate roles for the protein and mRNA nuclear export machineries in tRNA nuclear export. Mutations of genes encoding actin cytoskeleton components and mitochondrial outer membrane proteins also cause accumulation of unspliced tRNA, likely due to defective splicing on mitochondria. Additional gene products, such as chromatin modification enzymes, have unanticipated effects on pre-tRNA end processing. Thus, this genome-wide screen uncovered putative novel pathways for tRNA nuclear export and extensive links between tRNA biology and other aspects of cell physiology.
Citation Genes Dev. 2015; 29:2633-44

Datasets

Download the list of datasets
Papers Phenotype Conditions Collection Tested mutants Data Details
Wu J~Hopper AK, 2015 tRNA processed form 1/2 (tRNA(Ile)(UAU)) standard [standard] hap a 4,760 Discrete
Wu J~Hopper AK, 2015 tRNA processed form 1/2 + extra band below I (tRNA(Ile)(UAU)) standard [standard] hap a 4,760 Discrete
Wu J~Hopper AK, 2015 tRNA processed form 2* (tRNA(Ile)(UAU)) standard [standard] hap a 4,760 Discrete
Wu J~Hopper AK, 2015 tRNA processed form 4/5/6 (tRNA(Ile)(UAU)) standard [standard] hap a 4,760 Discrete
Wu J~Hopper AK, 2015 tRNA processed form 7 (tRNA(Ile)(UAU)) standard [standard] hap a 4,760 Discrete
Wu J~Hopper AK, 2015 tRNA processed form 9 (tRNA(Ile)(UAU)) standard [standard] hap a 4,760 Discrete

Curation history

Data

Sept. 20, 2016 Waiting for tested.
July 10, 2017 Ready to load.
Jan. 9, 2018 Loaded.

Tested strains

Sept. 20, 2016 To request.
May 10, 2017 Request sent.
July 10, 2017 Ready to load.
Jan. 9, 2018 Loaded.