Nislow C~Hammond TG, 2015

Pubmed ID 25667933
Title Genes required for survival in microgravity revealed by genome-wide yeast deletion collections cultured during spaceflight.
Authors Corey Nislow, Anna Y Lee, Patricia L Allen, Guri Giaever, Andrew Smith, Marinella Gebbia, Louis S Stodieck, Jeffrey S Hammond, Holly H Birdsall, Timothy G Hammond
Abstract Spaceflight is a unique environment with profound effects on biological systems including tissue redistribution and musculoskeletal stresses. However, the more subtle biological effects of spaceflight on cells and organisms are difficult to measure in a systematic, unbiased manner. Here we test the utility of the molecularly barcoded yeast deletion collection to provide a quantitative assessment of the effects of microgravity on a model organism. We developed robust hardware to screen, in parallel, the complete collection of ~4800 homozygous and ~5900 heterozygous (including ~1100 single-copy deletions of essential genes) yeast deletion strains, each carrying unique DNA that acts as strain identifiers. We compared strain fitness for the homozygous and heterozygous yeast deletion collections grown in spaceflight and ground, as well as plus and minus hyperosmolar sodium chloride, providing a second additive stressor. The genome-wide sensitivity profiles obtained from these treatments were then queried for their similarity to a compendium of drugs whose effects on the yeast collection have been previously reported. We found that the effects of spaceflight have high concordance with the effects of DNA-damaging agents and changes in redox state, suggesting mechanisms by which spaceflight may negatively affect cell fitness.
Citation Biomed Res Int 2015; 2015:976458

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) time [14 gen] hom 4,060 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) time [21 gen] hom 4,060 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) microgravity [unknown], time [14 gen] hom 3,995 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) microgravity [unknown], time [21 gen] hom 3,995 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) sodium chloride [0.5 M], time [14 gen] hom 4,010 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) sodium chloride [0.5 M], time [21 gen] hom 4,010 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) sodium chloride [0.5 M], microgravity [unknown], time [14 gen] hom 3,837 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) sodium chloride [0.5 M], microgravity [unknown], time [21 gen] hom 3,837 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) time [21 gen] het 2,803 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) microgravity [unknown], time [21 gen] het 5,145 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) sodium chloride [0.5 M], time [14 gen] het 2,745 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) sodium chloride [0.5 M], time [21 gen] het 2,745 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) sodium chloride [0.5 M], microgravity [unknown], time [14 gen] het 5,120 Quantitative
Nislow C~Hammond TG, 2015 growth (relative abundance in pooled culture) sodium chloride [0.5 M], microgravity [unknown], time [21 gen] het 5,120 Quantitative

Curation history

Tested strains

Oct. 11, 2016 Ready to load.
Dec. 9, 2016 Loaded.

Data

Oct. 11, 2016 Ready to load.
Dec. 9, 2016 Loaded.