Eide DJ~Harper JF, 2005

Pubmed ID 16168084
Title Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae.
Authors David J Eide, Suzanne Clark, T Murlidharan Nair, Mathias Gehl, Michael Gribskov, Mary Lou Guerinot, Jeffrey F Harper
Abstract Nutrient minerals are essential yet potentially toxic, and homeostatic mechanisms are required to regulate their intracellular levels. We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae. Using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), we assayed 4,385 mutant strains for the accumulation of 13 elements (calcium, cobalt, copper, iron, potassium, magnesium, manganese, nickel, phosphorus, selenium, sodium, sulfur, and zinc). We refer to the resulting accumulation profile as the yeast 'ionome'.
Citation Genome Biol. 2005; 6:R77
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Datasets

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Paper Phenotype Condition Reference Collection Tested mutants Data Details
Eide DJ~Harper JF, 2005 abundance of calcium standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of cobalt standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of copper standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of iron standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of magnesium standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of manganese standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of nickel standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of phosphorus standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of potassium standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of selenium standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of sodium standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of sulphur standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits
Eide DJ~Harper JF, 2005 abundance of zinc standard YPD + mineral supplement stock hom 4,380 Quantitative only for hits

Curation history

Tested strains
July 20, 2016 Request sent.
July 20, 2016 Ready to load.
Oct. 6, 2016 Loaded.

Data
July 20, 2016 To request.
July 21, 2016 Request sent.
Aug. 16, 2016 Request sent.
Sept. 20, 2016 Not available.
Sept. 20, 2016 Ready to load.
Oct. 6, 2016 Loaded.