Kwon Y~Koo JC, 2016

Pubmed ID 27510723
Title Signaling pathways coordinating the alkaline pH response confer resistance to the hevein-type plant antimicrobial peptide Pn-AMP1 in Saccharomyces cerevisiae.
Authors Youngho Kwon, Jennifer Chiang, Grant Tran, Guri Giaever, Corey Nislow, Bum-Soo Hahn, Youn-Sig Kwak, Ja-Choon Koo
Abstract Genome-wide screening of Saccharomyces cerevisiae revealed that signaling pathways related to the alkaline pH stress contribute to resistance to plant antimicrobial peptide, Pn-AMP1. Plant antimicrobial peptides (AMPs) are considered to be promising candidates for controlling phytopathogens. Pn-AMP1 is a hevein-type plant AMP that shows potent and broad-spectrum antifungal activity. Genome-wide chemogenomic screening was performed using heterozygous and homozygous diploid deletion pools of Saccharomyces cerevisiae as a chemogenetic model system to identify genes whose deletion conferred enhanced sensitivity to Pn-AMP1. This assay identified 44 deletion strains with fitness defects in the presence of Pn-AMP1. Strong fitness defects were observed in strains with deletions of genes encoding components of several pathways and complex known to participate in the adaptive response to alkaline pH stress, including the cell wall integrity (CWI), calcineurin/Crz1, Rim101, SNF1 pathways and endosomal sorting complex required for transport (ESCRT complex). Gene ontology (GO) enrichment analysis of these genes revealed that the most highly overrepresented GO term was "cellular response to alkaline pH". We found that 32 of the 44 deletion strains tested (72 %) showed significant growth defects compared with their wild type at alkaline pH. Furthermore, 9 deletion strains (20 %) exhibited enhanced sensitivity to Pn-AMP1 at ambient pH compared to acidic pH. Although several hundred plant AMPs have been reported, their modes of action remain largely uncharacterized. This study demonstrates that the signaling pathways that coordinate the adaptive response to alkaline pH also confer resistance to a hevein-type plant AMP in S. cerevisiae. Our findings have broad implications for the design of novel and potent antifungal agents.
Citation Planta 2016; 244:1229-1240

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Kwon Y~Koo JC, 2016 growth (relative abundance in pooled culture) Pn-AMP1 [3.2uM] hom 4,497 Quantitative
Kwon Y~Koo JC, 2016 growth (relative abundance in pooled culture) Pn-AMP1 [3.2uM] het 1,108 Quantitative

Curation history

Data

Oct. 17, 2016 To request.
Nov. 18, 2016 Request sent.
Nov. 23, 2016 Not available.
Nov. 23, 2016 Waiting for tested.
June 23, 2017 Ready to load.
Sept. 1, 2017 Waiting for tested.
Sept. 3, 2017 Ready to load.
Sept. 12, 2017 Loaded.

Tested strains

Oct. 17, 2016 To request.
Nov. 18, 2016 Request sent.
June 23, 2017 Request abandoned.
Sept. 1, 2017 Request sent.
Sept. 3, 2017 Ready to load.
Sept. 12, 2017 Loaded.