Gonzalez R~Valero E, 2016

Pubmed ID 27733850
Title Saccharomyces cerevisiae.
Authors Ramon Gonzalez, Pilar Morales, Jordi Tronchoni, Gustavo Cordero-Bueso, Enrico Vaudano, Manuel Quirós, Maite Novo, Rafael Torres-Pérez, Eva Valero
Abstract Saccharomyces cerevisiae, where the Hog1 stress activated protein kinase was discovered about 20 years ago. Hog1 is the core of the intracellular signaling pathway that governs the adaptive response to osmotic stress in this species. The main endpoint of this program is synthesis and intracellular retention of glycerol, as a compatible osmolyte. Despite many details of the signaling pathways and yeast responses to osmotic challenges have already been described, genome-wide approaches are contributing to refine our knowledge of yeast adaptation to hypertonic media. In this work, we used a quantitative fitness analysis approach in order to deepen our understanding of the interplay between yeast cells and the osmotic environment. Genetic requirements for proper growth under osmotic stress showed both common and specific features when hypertonic conditions were induced by either glucose or sorbitol. Tolerance to high-glucose content requires mitochondrial function, while defective protein targeting to peroxisome, GID-complex function (involved in negative regulation of gluconeogenesis), or chromatin dynamics, result in poor survival to sorbitol-induced osmotic stress. On the other side, the competitive disadvantage of yeast strains defective in the endomembrane system is relieved by hypertonic conditions. This finding points to the Golgi-endosome system as one of the main cell components negatively affected by hyperosmolarity. Most of the biological processes highlighted in this analysis had not been previously related to osmotic stress but are probably relevant in an ecological and evolutionary context.
Citation Front Microbiol 2016; 7:1545

Datasets

Download the list of datasets
Paper Phenotype Condition Medium Collection Tested mutants Data Details
Gonzalez R~Valero E, 2016 growth (relative abundance in pooled culture) Osmotic Stress-Sorbitol SD + His + Leu + Ura hom 4,144 Quantitative
Gonzalez R~Valero E, 2016 growth (relative abundance in pooled culture) High Osmostic Stress-Glucose SD + His + Leu + Ura hom 4,144 Quantitative

Curation history

Data

Oct. 17, 2016 Clarification needed.
Oct. 27, 2016 Request sent.
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Tested strains

Oct. 17, 2016 Clarification needed.
Oct. 27, 2016 Request sent.
Oct. 27, 2016 Ready to load.
Nov. 29, 2016 Loaded.