Hill SM~Nystroem T, 2016

Pubmed ID 27373154
Title Asymmetric Inheritance of Aggregated Proteins and Age Reset in Yeast Are Regulated by Vac17-Dependent Vacuolar Functions.
Authors Sandra Malmgren Hill, Xinxin Hao, Johan Grönvall, Stephanie Spikings-Nordby, Per O Widlund, Triana Amen, Anna Jörhov, Rebecca Josefson, Daniel Kaganovich, Beidong Liu, Thomas Nyström
Abstract Age can be reset during mitosis in both yeast and stem cells to generate a young daughter cell from an aged and deteriorated one. This phenomenon requires asymmetry-generating genes (AGGs) that govern the asymmetrical inheritance of aggregated proteins. Using a genome-wide imaging screen to identify AGGs in Saccharomyces cerevisiae, we discovered a previously unknown role for endocytosis, vacuole fusion, and the myosin-dependent adaptor protein Vac17 in asymmetrical inheritance of misfolded proteins. Overproduction of Vac17 increases deposition of aggregates into cytoprotective vacuole-associated sites, counteracts age-related breakdown of endocytosis and vacuole integrity, and extends replicative lifespan. The link between damage asymmetry and vesicle trafficking can be explained by a direct interaction between aggregates and vesicles. We also show that the protein disaggregase Hsp104 interacts physically with endocytic vesicle-associated proteins, such as the dynamin-like protein, Vps1, which was also shown to be required for Vac17-dependent sequestration of protein aggregates. These data demonstrate that two physiognomies of aging-reduced endocytosis and protein aggregation-are interconnected and regulated by Vac17.
Citation Cell Rep 2016; 16:826-38


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Papers Phenotype Conditions Collection Tested mutants Data Details
Hill SM~Nystroem T, 2016 mother:daughter asymmetric inheritance of protein aggregates (Hsp104-GFP) time [30 min], temperature [42ºC] hap a 3,882 Quantitative

Curation history

Dec. 19, 2016 Tested strains to request.
Dec. 19, 2016 Data clarification needed.
Feb. 1, 2017 Tested strains requested.
Feb. 1, 2017 Data requested.
April 25, 2017 Tested strains requested.
April 25, 2017 Data requested.
April 27, 2017 Tested strains to load.
April 27, 2017 Tested strains loaded.
April 27, 2017 Data to load.
April 27, 2017 Data loaded.